李晖
CASE DATAPatients, femade, 51 years old, worker, hospitalized in December1993. Chief complains: often dizziness complicated with vomiting,occasional numbness of fingers, disease course had been over 1year. Massage treatment was performed, but no effect wasachieved. Examination: cervical vertebrae located at center withoutlateral process, no obvious tenderness at bilateral neck muscles andno limitation of movement of cervical vertebrae. X-ray film showedhypertrophic changes of cervical vertebrae without obvious stenosisof intervertebral space. Diagnosis: cervical syndrome(sympathetictype) . Treatment: Wendan Tang Jiajian: astragalus root 20 g; big-head atractylodes rhizome 9 g; white peony root 9 g; Chuanduan12g; Chuanxiong 9 g; chaenomeles fruit 9 g; Fuling 12 g; Zhiqiao6g; Chao Zhuru; tangerine peel 6 g; Jiangbanxia, 9 g; Zhigancao 6g. 5tie(one tie per day) were administrated continuously until symptomsdisappeared. Patient searched medical service because of itching andno relapse of cervical syndrome was complained.
作者：周成谷 刊期： 2003年第06期
目的:以特定的细胞因子对其进行刺激和诱导,使已经体外分离并扩增的兔骨髓间充质干细胞向软骨细胞谱系分化,为半月板组织工程重建提供种子细胞.方法:将已分离并经体外传代培养扩增的兔骨髓间充质干细胞( mesenchymal stem cells, MSCs)在用碱性成纤维细胞生长因子( basic fibroblast growth factor, bFGF)和转化生长因子β1( transforming growth factor β1, TGF-β1)协同刺激后,用逆转录-聚合酶链式反应( reverse transcription polymerase chain reaction, RT PCR)的方法从分子水平寻找进入软骨细胞分化谱系的证据.结果:已分离并经体外扩增的兔骨髓 MSCs在用 bFGF和 TGF-β1协同刺激后,用 RT-PCR的方法探明有 pC I的编码基因表达,证明其已经进入软骨细胞分化谱系.结论: bFGF和 TGF-β1能够刺激 MSCs的体外增殖并且使其进入软骨细胞分化谱系,为半月板组织工程重建提供了可靠的自体来源种子细胞.
作者：徐青镭；吴海山；周维江；万年宇 刊期： 2003年第06期
AIM:To compare the effects of early enteral nutrition and early parenteral nutrition on ameliorating visceral ischemia and relieving free radical damage.METHODS:66 Wistar rats were divided into 3 groups: control group(C),parenteral nutrition group( PN) and enteral nutrition group( EN), PN and EN groups made up of 30% TBSAⅢ degree burn model.We delivered nutrient solution with same calorie and calorie- nitrogen ratio via vein or enteral tract respectively.Blood flow of liver,kidney and change of SOD of heart,liver and kidney at 6,12,24,48,72 h after burn were tested.RESULTS:Tissue blood flow and SOD of EN group were higher than those of PN group in many phase( P< 0.05-0.01) .CONCLUSION: Early enternal nutrition can relieve the increase of visceral vascular permeability and damage of oxygen free radical.
作者：徐世伟；冯怀志；尤忠义；王裴；汪仕良 刊期： 2003年第06期
INTRODUCTION Acute stress fracture which is also called as fatigue fracture is different from chronic stress fracture. Fracture and renovation co- exist during its pathologic process.All the patients selected for the study suddenly invasioned during overload locomotion exercise.Substance of bone was normal and had no repair phenomenon in the fracture.The purpose of this study was mainly to observe the curative effect of rehabilitation in postoperative fracture patients.
作者：陈庆贺；高吉昌；卢宇；晁民；王仑 刊期： 2003年第06期
TREATMENT EXPERIENCE FOR LUMBAR SPRAINMassage and traction are often used in treatment of lumbar sprainto alleviate adhesion of muscle and ligament, enlarge intervertebralspace. At the same time, local dialysis, physical therapy might beused, also acupuncture moxibustion. To massage treatment, painpoints at lumbodorsal or lumbosacral part are massaged for 15minutes, thereafter pressed for 10 minutes to promote lumbosacralblood circulation and excite himbosacral nerves. After pain, spasmare relieved, local compression of traditional Chinese medicine isperformed; Prescription includes: club-moss 15 g, burred tuber 12g, zedoary 12 g, large-leaf gentian root 12 g, ledeboriella root 9 g,erythrina bark 12 g, achyranthes root 10 g, chaenomeles fruit 10 g,safflower 10 g, Chinese angelica 10 g, homalomena rhizoma 12 g,sappan wood 10 g, decoted in water for compression on affectedsite.
作者：吴花梅；吴玲；刘岩 刊期： 2003年第06期
AIM:To study the regulation effect of different cytokines combinations on stem cell or progenitor of umbilical blood when no blood serum and matrix exist. METHODS: Collect and analyze the 23 sample of umbilical blood. RESULTS:(1) The combination of SCF+ Flk2/Flt3 ligand and( FL)± TPO can amplify CD34,+ ,CD34+ Thy- 1+ ,CD34+ CD33+ and LTC- IC in umbilical blood effectively and rapidly.(2) After add solubility type (sIL- 6R) of IL- 6 and IL- 6 receptor into this combination, the stem cell or progenitor proliferated prominently;When add IL- 6 alone(without sIL 6R), the content of CD34+ cells and LTC IC didn't increased obviously in early stage, but the amplification of CFU- Mix and BFU- E was not prominent as CFU- GM. (3)Detect apoptosis rate of cells by FITC- Annexin-Ⅴ labeled by membranous change in early stage of cellular apoptosis. After add Flt3L and /or IL- 6+ sIL- 6R, the Annexin-Ⅴ positive cells decreased from 15.2%～ 19.1% to 2.8%～ 3.5% . CONCLUSION: In suspending system composed with SCF+ TPO+ Flt3L+ IL- 6+ sIL- 6R without matrix and blood serum, the umbilical blood can not only produce large amount of committed progenitor but also keep certain quantitive hematopoietic cells of early stage.
作者：郭荣；何飞；孙明；陈波斌；林果为；周宏研 刊期： 2003年第06期
目的:明确影响椎间盘热疗( intradiscal electrothermal moclulation,IDET)治疗椎间盘源性下腰痛患者的相关因素.方法:通过对应用 IDET治疗的 50例椎间盘源性下腰痛患者术后平均 11个月( 6 ～ 19个月)的随访,采用对比手术前后的 VAS评分改变的方法进行资料分析,得出影响 IDET治疗效果的因素.结果:总有效率为 60%.年龄在 40岁以下者 (n=20)术后 VAS平均减少了 33%,优于 40岁以上者 (P< 0.001);非吸烟者 (n=28)优于吸烟者 (P< 0.0001);病程在 4年以内者 (n=39)优于 4年以上者 (P< 0.001);椎间盘退变轻者(改良 Dallas分型: I、 II、 III型)优于退变重者 (P< 0.001),加热导丝在椎间盘内的位置亦为重要的影响因素 (P< 0.0001).结论 :应用 IDET治疗椎间盘源性下腰痛患者时,在如下情况下将能获得更好的疗效: (1) 年龄小于 40岁; (2)病程在 4年以内; (3)非吸烟者; (4)椎间盘退变轻者(改良 Dallas分型: I、 II、 III型); (5)撕裂的后部纤维环能够全部被加热导丝加热者.
作者：刘保卫；张蒲；Hansen A Yuan 刊期： 2003年第06期
INTRODUCTIONSelf-prevention taught to patients by example and instruction has anidentified effect with high good rate and shortening therapeuticcourses, consolidating therapeutic effect and preventing relapse wereachieved.
作者：荆兆峰；孙西周；刘磊 刊期： 2003年第06期
目的: H反射是脊髓学突触反射,为探讨其影响因素,在 SD大鼠后肢 H反射模型上观察中药强力生注射液对该反射各参数的影响.方法:用电刺激诱发大鼠后肢 H反射,观察生理盐水组、实验组以及阳性对照组注射前后该反射各参数的变化.结果:生理盐水组注射前后无明显改变,实验组 H/M降低( P< 0.05),阳性对照组 H波潜伏期明显缩短( P< 0.01).结论:提示强力生可能通过调节α神经元影响神经-肌肉兴奋性,与士的宁的兴奋剂作用有本质区别.
作者：姜文凯；吕荣；倪正；华兴邦 刊期： 2003年第06期
中医手法治疗肌肉、关节的无菌性炎症具有止痛迅速无副作用的特点,其基本手法以顺肌纤维走行于条索状物处按揉、点压、弹拔为主.需要注意的是治疗前应排除内脏器质性病变.
作者：范剑非；郭思宁 刊期： 2003年第06期
INTRODUCTION Genesis and advance of tumor includes excessive multiplication and less apoptosis.Research on apoptosis contributes to revealing biological characteristics of tumor and proving data for exploring of genesis mechanism,diagnosis and treatment.Bcl- 2 family is a commonly studied apoptosis- related gene family.Bax is the main gene promoting apoptosis in bcl- 2 family and its expression level is low in most tumor.But some scholars considered that expression level of bax protein is higher in some kinds of tumor.Bcl- w has been considered to have antiapoptosis function,but corresponding reports are very few.In this research,expression levels of bax and bcl- 2 were determined by imumohistochemistry to find its correlation with clinical pathological characteristics.
作者：吴国强；徐宏勇；李开宗；窦科峰 刊期： 2003年第06期
目的:通过对眼烧伤新西兰白兔和眼烧伤患者应用含药辐照异种角膜后的实验研究及临床观察, 论证含药辐照异种角膜治疗眼烧伤的疗效, 探索出一种理想的眼烧伤治疗方法.方法:将 36只酸、碱眼化学烧伤的新西兰白兔随机分为实验组和对照组,实验组应用含药辐照异种角膜治疗,对照组按常规治疗.比较实验组和对照组的伤眼感染、角膜溃疡及角膜穿孔发生率.将 55例( 88眼)眼烧伤患者随机分为治疗组和对照组,治疗组应用含药辐照异种角膜治疗,对照组按常规治疗.比较治疗组和对照组的伤眼治愈率.结果:动物实验:细菌培养无菌生长,体外抑菌试验显示含药辐照异种角膜对绿脓杆菌和金黄色葡萄球菌均有很强的抑菌活性,实验组伤眼感染率及角膜溃疡、穿孔率远低于对照组,两组比较差异有显著性意义( P< 0.05).临床研究表明治疗组治愈率远高于对照组,两组比较差异有显著性意义 (P< 0.05).结论:含药辐照异种角膜完全达到无菌和较强抗菌活性的要求,具有促进角膜上皮愈合、防治感染和减少并发症的作用.
作者：肖长辉；易敬林；张红艳；曾元临；吴志宏；李国辉；詹剑华；辛国华 刊期： 2003年第06期
选择 109例临床患者,研究臂从神经阻滞联用关节松动术治疗肩周炎的临床效果.病例随机分为治疗组和对照组,治疗组 58例采取关节松动术联合应用臂从神经阻滞治疗;对照组 51例采用臂从神经阻滞治疗.定期观察治疗后两组患者的临床症状 ,体征及肩关节活动度的改善. 治疗结果显示两组治疗效果、治疗次数比较,治疗组明显优于对照组,经统计学处理,差异有显著性意义( P< 0.05).研究提示,关节松动术联用臂从神经阻滞治疗肩周炎是一种较有效的临床治疗措施.
作者：施宇翔；熊利泽；计根林；易南；黄卫民 刊期： 2003年第06期
0 引言 中波紫外线辅助治疗带状疱疹的效果不同于单纯药物治疗的效果,为此收集了有关资料并作分析.
作者：余红；凌波；凌亚君；钱黎华 刊期： 2003年第06期
0 引言 骨欣肽、谷慷泰灵注射液广泛用于临床骨科术后、股骨头坏死等骨科疾病治疗.普乐林注射液可改善髋部骨折患者下肢血流淤滞状态,从而预防长久无卧床已发生的下肢静脉血栓及肺栓塞等 [1].目前一些注射液与输液配伍后不溶性微粒增加,造成局部血管堵塞供血不足,产生静脉炎和水肿、肉芽肿, 过敏反应、热原样反应 [2].本实验研究以上 3种注射液与 4种不同输液配伍后微粒变化情况.
作者：张靖贤 刊期： 2003年第06期
AIM:To explore the co- effect of nerve growth factor (NGF) and basic fibroblast growth factor (bFGF) on the sciatic nerve after injury. METHODS: 96 rats were divided into normal saline group, NGF group, bFGF group and NGF+ bFGF group. Sciatic nerve function index (SFI), nerve conduction velocity, regeneration axon counting were detected in the 3 rd, 6 th, 9 th, 12 th week postinjury. RESULTS:The end of SFI, nerve conduction velocity, regeneration axon counting in NGF+ bFGF group were higher than those in the other groups. CONCLUSION:The co- effect of NGF and bFGF on sciatic nerve is better than the effect of NGF or bFGF alone.
作者：梁崇礼；赵稳兴 刊期： 2003年第06期
BACKGROUND: To patients with burn of face, how to preventand reduce facial deformity in maximal degree is an important sub-ject in burn iatreusiology.
作者：刘海霞；肖康菊；黄贤慧 刊期： 2003年第06期
0 引言 临床上常遇到儿童颅脑损伤患者接受高压氧治疗的情况,对这类患者的治疗和护理程序均根据医生的意见制定,对出现的一些问题一般根据以往临床经验处理或请专家会诊. 2001年试运用循证方法解决小儿颅脑损伤高压氧治疗中存在的问题,为制定对患儿有益的治疗、护理方案提供依据.
作者：李静艳 刊期： 2003年第06期
目的:探讨老年髋部骨折围术期全方位护理对其日后功能恢复的作用.方法:患者入院时即开始全方位护理.制订护理计划:术前:肌体应激训练,术后机能适应性训练,牵引期训练;术后:术后早期护理,术后功能恢复训练.结果:随访 0.5～ 2.0年,功能恢复优者占 79%.结论:全方位护理使患者具有充分的心理准备和良好的机体条件,防止可能发生的并发症,尽快恢复生理功能.
作者：黄芳艳 刊期： 2003年第06期
目的:神经干细胞的发现为神经系统退行性疾病的治疗,提供了新的方法.通过碱性成纤维细胞生长因子( basic fibroblast growth factor,bFGF)反应性神经干细胞的移植 ,探讨神经干细胞在帕金森病 (Parkinson'disease,PD)治疗中的作用.方法:体外用 bFGF作为丝裂原培养 E16大鼠中脑神经干细胞,经 BrdU标记后植入 PD大鼠纹状体,了解 PD大鼠旋转行为的改善情况.结果:神经干细胞移植组 PD大鼠旋转行为改善明显.结论: bFGF反应神经干细胞移植对 PD有明显的治疗作用.
作者：阴金波；周政；杨辉；张可成；宋业纯 刊期： 2003年第06期
中国组织工程研究杂志
主管:中华人民共和国卫生部
主办：中国康复医学会,《中国组织工程研究与临床康复》杂志社
