目的:探讨大鼠肾缺血再灌注(IR)损伤不同时间c-Fos、Bcl-2、ICAM-1蛋白的表达及地塞米松(Dex )和川芎嗪(TMPz )对其影响.方法:用免疫组化法检测大鼠急性肾缺血再灌注不同时间内及Dex、TMPz干预后c-Fos、Bcl-2、ICAM-1蛋白表达的分布及强度变化.结果:c-Fos蛋白分布于近曲小管、远曲小管、集合管上皮细胞的细胞核、细胞浆内,再灌注后1 h表达明显增强,3 h达高峰,6 h锐减.Bcl-2蛋白主要分布于近曲小管上皮细胞的细胞浆,再灌注后1 h表达明显增强,6 h达高峰,24 h仍有较强表达.ICAM-1蛋白分布在肾血管、肾小管等部位,其中以肾血管为著,其表达增强于再灌注后1 h,直到24 h表达仍有增强.Dex +TMPz +IR组,Dex+IR及TMPz+IR组c-Fos、ICAM-1蛋白表达明显低于IR组(P＜0.01), Bcl-2表达则明显高于IR组(P＜0.01).结论:地塞米松和川芎嗪可能通过诱导Bcl-2蛋白的合成,下调c-Fos、ICAM-1蛋白的合成,减轻肾损伤.
作者：祝海洲；詹自力；何卫阳；苟欣 刊期： 2004年第05期
目的:探讨蚯蚓蛋白激酶制剂( LK)的纤溶增强作用.方法:采用静脉给药方法,观察LK对SD大鼠纤溶激活因子t-PA及其抑制物PAI-1的急性作用.结果:给药后,PAI-1活性被显著抑制(P＜0.01),t-PA活性则明显增强(P＜0.01).剂量1 200 U/kg体重的LK,其增强t-PA作用显著大于600 U/kg体重剂量LK(P<0.05).此外,体外观察表明:LK具有明显的激活纤溶酶原(Plg)作用,且有量-效关系,具有一定的浓度依赖性(1.56-25 kU/L).结论:结果表明,LK具备增强纤溶的特性,静脉给药作用快速而肯定.
作者：张津；刘汇波；吴骋；樊蓉；周茗 刊期： 2004年第05期
NAD(P)H oxidase was initially found in phagocytes and it participates in the generation of reactive oxygen species(ROS). Recent researches have showed that NAD(P)H oxidase also expresses in other tissues including blood vessels and it plays a critical role in vascular remodeling through ROS which are important signaling molecules in vascular cells.This article reviews the biochemical characterization, activation paradigms, structure, and function of this enzyme.
作者：陈闻东；高平进；朱鼎良 刊期： 2004年第05期
The phenomenon of RNA interference (RNAi) is highly conserved mechanism in the organism evolution. As a immune system ,RNAi is a ubiquitous mechanism against invading microorganism in plant and animal cells. Recently, it has been found that RNAi is the process by which double-strand RNA(dsRNA) directs sequence-specific degradation of messenger RNA and the mediations of sequence specific messenger RNA degradation are 21-and 23-nucleotide small interfering RNAs that generate by ribonuclease from endogenous longer dsRNA or by transfectious technics from heterologous dsRNA. Over the past few years, the way in which cells respond to dsRNA by silencing homologous genes has revealed a new regulating paradigm in biology.
作者：孔祥平；LiXing W.Reneker 刊期： 2004年第05期
Endothelial progenitor cell (EPC) is a kind of directional cell that is able to differentiate to endothelial cell. The role of EPC is associated not only with vasculogenesis during embryonic development but also with physiological organ maintenance and angiogenesis during postnatal and adult period. There is a good clinical therapeutic prospective use for EPC in the treatment of ischemia diseases and inhibition of tumor angiogenesis.
作者：薛丽京；周序珑 刊期： 2004年第05期
Chloride channels distribute widely in the body, and participate in many physiological actions and regulatory processes. Based on their physiological roles and molecular structures, six kinds of chloride channels have been identified: (1) The chloride channels family; (2) Cystic fibrosis transmembrane conductance regulator; (3) Swelling-activated chloride channels; (4) Calcium-activated chloride channels; (5) The p64 (CLIC) gene family; (6) γ-aminobutyric acid and glycine receptors. The chloride channels do exist in platelets, and their appearances are dependent on the presence of intracellular calcium. Blocking agents of chloride channels inhibit the thrombin-activated platelet aggregation and the elevation of the intracellular calcium concentration in a dose-dependent manner. It is suggested that chloride channels play a role in the activation of platelets. In addition, chloride channels act on both the cell volume regulation and the intracellular pH regulation in platelets.
作者：陈晓琳；尹松梅 刊期： 2004年第05期
华中科技大学同济医学院基础医学院病理生理学系开展了病理生理学课程教学改革.取得的结果表明,知识积累-临床强化-科研创新是病理生理学有效可行的教学体系.
作者：刘声远；王建枝；曾金华；王迪浔；董时富；叶仕桥；刘蓉；方征宇；余上斌；陈琪玲；张少华 刊期： 2004年第05期
