张向群
腰椎间盘突出症是为常见的一种腰腿痛[1].为探索简便易行,治愈率高的非手术疗法,自1996年以来,我们采用口服药酒配合推拿治疗腰椎间盘突出症患者116例,取得了良好的效果,现报告如下.
作者:乔国一;王强;邵宗军 刊期: 2002年第24期
目的通过观察不同年龄增生性瘢痕与正常皮肤组织中转化生长因子β1(TGFβ1)的表达,初步探讨TGFβ1的表达与增生性瘢痕形成的年龄因素的关系.方法以增生性瘢痕为研究对象,正常皮肤组织为对照,采用SABC免疫组化方法观察1~19岁、20~50岁增生性瘢痕与各年龄组正常皮肤的细胞因子TGFβ1的表达.结果增生性瘢痕组织的TGFβ1含量显著大于正常皮肤.1~19岁增生性瘢痕组TGFβ1表达较20~50岁增生性瘢痕组的增高差异有具显著性意义(P<0.01).胎儿组皮肤TGFβ1表达低于正常皮肤(P<0.01).结论TGFβ1表达增高是增生性瘢痕形成的原因之一.
作者:邱林;金先庆;向代理;付跃先;田晓菲 刊期: 2002年第24期
下肢永久性假肢装配后,步态训练是重要的.在实际训练中,患者穿戴下肢永久性假肢的步行模式应该尽可能接近于正常人的步行模式.文章着重讨论了下肢永久性假肢装配后的功能训练和步态分析方法.以期在临床工作中更好的发挥假肢的替代功能.
作者:瓮长水 刊期: 2002年第24期
Background: In recent years, success rate of replantion of severed finger is increased every year with developing of fiber surgery. But postoperative local vascular spasm and hypercoagulability often leads to the incidence of thrombosis and circulatory crisis that were an important reason leading to operation failure.
作者:朱爱平 刊期: 2002年第24期
由于长期药物治疗效果有限,且会带来副作用(例如反应性波动,神经症状,多巴胺诱导的运动障碍),同时神经影像技术的发展,使得对基本神经节功能结构的深入了解以及趋实质性技术的发展,引起了人们对外科治疗晚期帕金森病(PD)的兴趣.功能性的趋实质性神经外科治疗在晚期PD患者的治疗中日益显示出其重要性.发明苍白球手术治疗PD虽只有10年,但已取得一定的进步.
作者:朱虹;李鉴峰 刊期: 2002年第24期
作者: 刊期: 2002年第24期
Most of patients with intertrochanteric fracture of femur were treated by operations, such as Duogensi needle,Jewett pin,AO angle steel plate, Gamma pin and Richards pin. We treated 28 cases of intertrochanteric fracture of the femur with dynamic condylar screw from January 1997 to January 2000. Report as follow:
作者:洪加源;康两期;郭林新;练克俭;丁真齐;郭延杰;翟文亮;郭志民 刊期: 2002年第24期
作者: 刊期: 2002年第24期
Background:If patients with temporomandibular joint disease can't keep exercise of opening mouth,or such factors as wound injection,insertional shift occurs,which can lead to postoperative recurrence.
作者:李晓青;刘蕊 刊期: 2002年第24期
目的该研究通过对兔腓总神经传导速度等多项目的观察,探索低能量氦-氖激光对周围神经再生的影响.方法用44只体重2.5kg左右的家兔随机分为4,8,12及16周4个观察组,照射组各用兔6只,对照组各用5只.麻醉后,均切断左侧腓总神经,用9/0尼龙单丝对端吻合神经外膜.照射组在术后1天开始用8318B型低能量氦-氖激光仪经皮肤照射L5~6脊髓节段,每天照射15min,共照射14d.对照组不照射,均按期观察.结果术后4周,可在照射组看到细小而稀少的再生轴突,对照组直到术后8周才能看到(P<0.01).照射组的腓总神经运动神经传导速度均优于对照组(P<0.01),动作电位波幅照射组也优于对照组.胫前肌肌纤维横纹,照射组16周时很清楚,对照组不甚清楚.展趾功能到术后16周时,照射组与健侧相同,对照组才恢复到照射组12周的水平.结论低能量氦-氖激光促进了脊髓运动神经细胞功能,加速了轴突再生,促进神经修复后肢体功能的恢复.
作者:徐新智;吕荣;王军;张慧杰 刊期: 2002年第24期
假肢装配是个非常复杂的人-机-环境相结合的过程.截肢者需要终生使用假肢.假肢装配后,穿戴在截肢者身上进行反复的适合检查、修改、调整是保证假肢装配质量,尽量减少长期使用假肢引起并发症的重要方法.假肢适合检查分为初检、终检两个阶段.初检的假肢是初步组装的半成品,便于修改、调整,应当反复检查,反复修改.终检的假肢是已完成的产品.文章介绍了小腿假肢、大腿假肢适合检查的主要内容,检查方法、基本要求和常见问题.
作者:赵辉三 刊期: 2002年第24期
Objective To study the changes and significance of neuron specific enolase(NSE) and glial fibrilous acidic protein(GFAP) in rat cerebral concussion.Methods 80 Wistar male rats were used for animal model of cerebral concussion,which were sacrificed on the 1st,3rd, 7th,14th and 30th days after injury and the brain tissue were taken off.The expressions of NSE and GFAP were studied in the course of cerebral concussion by means of immunohistochemistry. Results Rats in 100 g group were seen the clinical manifestation for typical concussion.The pathologic changes were the cerebral vascular constriction and dilation,congestion and edema of cerebral tissue and neuronal degeneration and necrosis.NSE was increased on the 1st day,and the positive area was seen in the plasma of the neurons in the cerebral cortex and the cerebellum,and also seen in blood vessels,cerebrospinal fluid in aqueduct and interstitial matrix.NSE was obtained at peak on the 7th day,decreased on the 14th day and still raised on the 30th day.GFAP was increased on the 1st day,which the positive area was seen in the plasma of astrocytes,and obtained at peak on the 3rd day,which fiber like GFAP was in short,thick and astrocytes increased.GFAP decreased on the 7th day and obtained normal level in 30 days.Conclusion The main pathologic changes of cerebral concussion were blood circulatory disorder and nervous cells degeneration,apoptosis and necrosis.NSE and GFAP participated in the course of cerebral concussion,may play an important role in the damage of blood brain barrier, nervous cells degeneration and necrosis.
作者:彭瑞云;高亚兵;王德文;肖兴义;陈浩宇;吴小红;刘杰;胡文华;蔡宝仁;薛官生;张援平;尹晓梅 刊期: 2002年第24期
This article summarized exposure area, treatment of mental nerve, soft tissue stalk, osteotomy position and fixation method. 1 Mental part exposure area and treatment of mental nerve.
作者:刘妍琼;华泽权 刊期: 2002年第24期
目的探讨外源性人端粒酶蛋白催化亚单位(hTERT)基因转染对人胚胎成纤维细胞(hEFs)寿命的影响以及基因转染后细胞是否存在恶性表型.方法应用脂质体法将pIRES2EGFPhTERT正义重组质粒及pIRES2EGFP空载质粒分别导入原代培养hEFs,Westernblot检测hTERT蛋白及I、III型胶原表达.染色体核型分析、裸鼠皮下致瘤性实验、DNA倍体实验以及形态学观察分析转染细胞是否存在恶性表型.结果外源性hTERT基因转染的胚胎成纤维细胞hTERT蛋白和I、III型胶原表达增加,体外传代次数增加,传至75代仍保持以2d的倍增周期生长,并且染色体仍为23对,均为正常二倍体细胞;裸鼠皮下不具有成瘤的能力;而hEFs和空载转染细胞传至60代左右平均五六天传代1次,出现生长停滞现象.结论外源性hTERT基因转染使胎儿成纤维细胞增殖旺盛,寿命延长,且不具有恶性表型.
作者:梁光萍;罗向东;杨宗城 刊期: 2002年第24期
目的探讨正常皮肤、增生性瘢痕、瘢痕疙瘩周边部和中央部及瘢痕疙瘩周围皮肤(距瘢痕疙瘩边缘0.5cm范围内)的成纤维细胞呈现不同生物学性状的细胞生物学机理.方法取手术切除的正常皮肤、增生性瘢痕和瘢痕疙瘩组织及周围皮肤各6例为标本,通过细胞培养6~10代后,应用流式细胞仪不同来源的成纤维细胞细胞周期的分布,同时比较它们在无血清培养条件下和在不同浓度FasMcAb作用下的细胞凋亡率.MTT比色法比较其细胞增殖活性.结果正常皮肤成纤维细胞增殖及凋亡正常.增生性瘢痕增殖活跃且细胞凋亡状况不良,瘢痕疙瘩中央部成纤维细胞处于低增殖-不凋亡状况;瘢痕疙瘩周边部成纤维细胞处于高增殖-无凋亡状况;瘢痕疙瘩周围正常皮肤大量细胞也处于高增殖及低凋亡状况.结论病理性瘢痕成纤维细胞增殖凋亡状况的异常可能是导致其不同生长特性的细胞生物学机理之一.
作者:鲁峰;高建华;段红杰 刊期: 2002年第24期
1 GENERAL DATA Case condition:Famle,aged 35 years old,craninal bone repair was performed on May 1998.deficiency of cranial 5 cm× 5 cm,region:tempora and parietal area.Operative time:type of operation is II type repair,repair material was fixed with double size 7 silk,and moulded under local intensified anesthesin.Wound were duched with gentamycin sulfate liquid,drain was laid,removed on next day.Secondary operation:Result of exploration(leading to pain):subcutaneous hydrops.
作者:黎铁伟 刊期: 2002年第24期
Objective To observe influence of fibrin sealant(FS) on osteoinductive ability of inject type BMP.Method The inject type BMP power was dissolved in the main glue part or thrombin part of FS, then mixed with the main glue part or thrombin part of FS into gel, observe coagulating time, then implant composite into the thigh muscle pouch of mice to evaluate their capacity to induce new bone formation, and compared to the single BMP implant group.Result There was no difference in the coagulating time between two mixing method, the osteoinductive ability of implants BMP dissolved in the main glue part or thrombin part of FS group was higher than that of simply BMP implant group.Conclusion FS was perfect carrier to inject type BMP.
作者:王登虎;刘建;李丹;胡蕴玉;袁志 刊期: 2002年第24期
Background: Femoral subtrochanteric fracture means fractures happened 5 cm away from inferior margin level of small trochanter. Most of this kind of fracture is unstable fractures caused by trauma mostly and characterized with large malposition, splintered and difficult to be treated. It will lead to a series of severe complications if treated improperly.
作者:周峰;姜为民;唐天驷;杨惠林 刊期: 2002年第24期
目的探讨颈性冠心病的推拿康复治疗疗效.方法选择92例随机分为推拿康复治疗组与药物对照组.结果显示总有效率91.3%,与对照组相比差异有显著性意义(P<0.05).结论推拿治疗颈性冠心病具有较好的效果.
作者:毛忠清;黄烟辉;许卫平;许仕纳 刊期: 2002年第24期
目的探讨人重组骨形成蛋白-2(rhBMP2)对人骨髓成骨细胞增殖和分化的影响以及剂量效应关系.方法以改良方法培养的骨髓成骨细胞为作用底物,加入梯度浓度的rhBMP2溶液,应用MTT法和碱性磷酸酶(ALP)染色法检测其对细胞增殖和ALP活性的影响.结果rhBMP2浓度低于1μg/ml时对人骨髓成骨细胞的增殖无明显影响,高于此浓度时对细胞的增殖有明显的促进作用,但无显著的剂量效应关系.从0.1~10μg/ml,ALP与rhBMP2具有剂量依赖性促进关系,rhBMP2浓度为10μg/ml时,其ALP活性是对照组的4倍,在高于10μg/ml时ALP活性并没有进一步显著提高.结论rhBMP2对骨髓成骨细胞的增殖和碱性磷酸酶活性具有促进作用,其佳效应浓度为10μg/ml.
作者:卢丙仑;冯志华;刘宝林;洪咏龙;汤苏阳 刊期: 2002年第24期