癌症生物学与医学（英文版）杂志

脂质体介导入钠/碘同向转运体基因转染肺癌A549细胞及其蛋白表达

目的:研究人钠/碘同向转运体(NIS)基因转染肺癌细胞及其蛋白表达.方法:将体外培养的肺癌A549细胞分为实验组和对照两组:以脂质体Lipofectamine2000为载体,分别介导转染重组质粒pcDNA3-hNIS和空质粒pcDNA3.NIS基因重组质粒pcDNA3-hNIS进行扩增、纯化,并经酶切鉴定和DNA测序.采用Western Blot法和免疫组化法分别检测转染肺癌细胞中NIS蛋白表达.结果:酶切鉴定年:UDNA测序结果表明重组质粒pcDNA3-hNIS中插入的hNIS基因片段大小、方向正确.Western Blot法和免疫组化染色结果显示实验组有NIS蛋白表达,其阳性率达70.6%且主要分布于细胞膜而对照组无表达.两组比较有显著性差异(P=0.000).结论:脂质体Lipofectamine 2000介导转染人钠/碘同向转运体基因pcDAN3-hNIS肺癌细胞能够成功地表达MS蛋白.

作者：严煜；张宏飞；张裕东；王晓谭 刊期： 2008年第01期

乳腺癌前病变和乳腺癌中α、γ-tubulin蛋白表达的免疫荧光定量分析及其意义

目的:研究乳腺导管上皮不典型增生(ADH)、导管内癌(DCIS)及浸润性导管癌(IDC)中α-tubulin和г-tubulin表达的变化及其意义,进一步探讨中心体异常与乳腺癌发生发展的相关性.方法:研究样本被分为导管上皮不典型增生、导管内癌和浸润性导管癌3组,每组30例.采用流式细胞免疫荧光定量分析技术和免疫组织化学方法,检测上述各组中心体微管蛋白α-tubulin和г-tubulin表达水平,与30例正常乳腺组的检测结果比较,并进行统计学分析.结果: 在全部样本组中гα-tubulin及г-tubulin FITC标记的阳性细胞数均数不同,差异有显著性(P=0.000),浸润性导管癌组的过表达高,正常组织组低.α-tubulin、г-tubulin表达水平随着细胞增殖级别的增加和分化程度的变差呈增高趋势.除不典型增生组和导管内癌组两组间比较以外,其余各组间差异均有显著性(P=0.001).在每组中α-tubulin和г-tubulin的表达程度无统计学差异(P＜0.05),显示免疫荧光定量分析和免疫组织化学检测结果趋于一致.结论:乳腺癌发生的早期阶段即存在着中心体蛋白的过度表达,推测这种异常表达在促进细胞恶性转化、形成乳腺癌过程中可能扮演了重要角色.对于中心体蛋白过表达的检测,免疫荧光定量和免疫组化两种技术可相互补充.

作者：牛昀；张彤文；王颍；韦丽；魏锡胤；牛瑞芳 刊期： 2008年第01期

作者： 刊期： 2014年第01期

Objective:Various nanoparticles have been designed and tested in order to select optimal carriers for the inhalation delivery of anticancer drugs to the lungs.Methods:hTe following nanocarriers were studied:micelles, liposomes, mesoporous silica nanoparticles (MSNs), poly propyleneimine (PPI) dendrimer-siRNA complexes nanoparticles, quantum dots (QDs), and poly (ethylene glycol) polymers. All particles were characterized using the following methods:dynamic light scattering, zeta potential, atomic force microscopy, in vitro cyto-and genotoxicity. In vivo organ distribution of all nanoparticles, retention in the lungs, and anticancer effects of liposomes loaded with doxorubicin were examined in nude mice atfer the pulmonary or intravenous delivery.Results:Signiifcant differences in lung uptake were found atfer the inhalation delivery of lipid-based and non-lipid-based nanoparticles. hTe accumulation of liposomes and micelles in lungs remained relatively high even 24 h atfer inhalation when compared with MSNs, QDs, and PPI dendrimers. hTere were notable differences between nanoparticle accumulation in the lungs and other organs 1 and 3 h atfer inhalation or intravenous administrations, but 24 h atfer intravenous injection all nanoparticles were mainly accumulated in the liver, kidneys, and spleen. Inhalation delivery of doxorubicin by liposomes signiifcantly enhanced its anticancer effect and prevented severe adverse side effects of the treatment in mice bearing the orthotopic model of lung cancer.Conclusion:hTe results of the study demonstrate that lipid-based nanocarriers had considerably higher accumulation and longer retention time in the lungs when compared with non-lipid-based carriers atfer the inhalation delivery. hTese particles are most suitable for effective inhalation treatment of lung cancer.

作者： 刊期： 2014年第01期

Objective:To analyze the clinicopathologic characteristics and prognostic factors of small gastrointestinal stromal tumor (GIST) of the stomach.Methods:A total of 31 small gastric GIST patients, including 10 males and 21 females, with a median age of 58 years (37-81 years), who underwent surgery at any time from 1999 to 2012 were included in this study. The clinical records of the patients were analyzed retrospectively.Results:Abdominal discomfort and pain (10 cases, 32.3%, respectively) were the two most common complaints among the patients. All patients received surgery, 11 received gastric wedge resection, 11 received subtotal gastrectomy, 5 received laparoscopic gastric wedge resection, and 4 received endoscopic submucosal dissection. No severe adverse complication was observed. A total of 29 patients (93.5%) were followed up. During the follow-up, 2 patients were found to exhibit tumor recurrence, and 1 patient had liver metastases. One patient died of tumor progression, while another died of another malignant tumor. Median progression free survival (PFS) time was 120.3 months, and median overall survival (OS) time was 130.4 months.Conclusion:Small gastric GIST has better prognosis. Surgery is the best choice for therapy. Micro-invasive procedures are safe and effective for elective patients. Tumor necrosis, tumor bleeding, and muscle invasion are potential prognostic factors of small gastric GIST.

作者： 刊期： 2013年第03期

作者： 刊期： 2013年第03期

高迁移率族蛋白HMGB1在宫颈鳞癌组织中的表达

目的:探讨宫颈鳞癌组织中高迁移率族蛋白HMGB1表达情况及其与肿瘤分化程度、大小、浸润和转移的关系.方法:应用组织芯片免疫组织化学方法检测了30例宫颈原位癌,90例无转移的宫颈癌,30例有转移的宫颈癌和30例正常宫颈鳞状上皮中HMGB1表达情况,并应用western blot技术定量检测其表达情况.结果:HMGB1在宫颈鳞癌组织中呈强阳性表达58.7%(88/150),正常宫颈鳞状上皮中弱表达,差异具有显著性(P＜0.01),HMGB1阳性率与肿瘤的大小、浸润及转移呈正相关(P＜0.01);与肿瘤分化程度无关(P＞0.05).结论:HMGB1在宫颈鳞癌组织中尤其是发生转移的宫颈鳞癌组织中呈强阳性表达,可作为宫颈鳞癌浸润、转移及预后的重要判定指标之一,并有望成为基因治疗的靶点.

作者：付欣；杜晓琴；郝权 刊期： 2008年第01期

比亚芬防治鼻咽癌同期调强放疗和化疗放射性皮肤损伤的疗效观察

目的:观察比亚芬乳膏防治鼻咽癌同期调强放疗及化疗患者急性放射性皮肤损伤的临床疗效.方法:将51例需要放射治疗的患者随机分为2组,均采用瓦里安600CD含120叶多叶光栅的6MV X线三维适形调强技术照射,并联合卡培他滨同步化疗.25例在第1次放射治疗及化疗后给予比亚芬乳膏涂抹为用药组;26例只做常规放射治疗宣教和健康教育为对照组.结果:两组患者皮肤反应发生率为100%.轻度放射反应(Ⅰ+Ⅱ度):用药组为88.0%(22/25例),对照组为57.7%(15/26例);Ⅲ度放射反应:用药组12.0%(3/25例),对照组42.3%(11/26例).两组之间有显著差异P＜0.01.皮肤反应发生剂量:用药组32.0%(8/25例)、对照组96.2%(25/26例),出现于照射40G y以前.结论:比亚芬能有效地预防鼻咽癌放、化疗同期较严重放射性皮炎的发生,对患者能减轻痛苦、提高生话质量、保证放射治疗的顺利进行.

作者：王若峥；吾甫尔；王多明；刘凯 刊期： 2008年第01期

Breast metastasis from extra-mammary malignancy is rare. An incidence of 0.4% to 1.3% has been reported in literature. hTe primary malignancies that most commonly metastasize to the breast are leukemia, lymphoma, and malignant melanoma. In this report, two cases of pulmonary metastasis to the breast were presented. A 40-year-old female manifested a right breast mass of 2-month duration. Atfer physical examination was performed, a poorly deifned mass was noted in the upper outer quadrant of the right breast. Another 49-year-old female manifested right breast mass of 5-day duration. A poorly deifned mass was noted in the lower inner quadrant of the right breast. Mammography results also revealed breast cancer. hTe patients underwent local excision. Atfer histological and immunohistochemical analyses were conducted, a primary lung carcinoma that metastasized to the breast was diagnosed. An accurate differentiation of metastasis to the breast from primary breast cancer is very important because the treatment and prognosis of the two differ signiifcantly.

作者： 刊期： 2014年第03期

Objective:To investigate the uptake rate of prostate speciifc antigen (PSA) testing among Hong Kong Chinese males aged 50 or above, and identify factors associated with the likelihood of undergoing a PSA test.Methods:A population-based telephone survey was conducted in Hong Kong in 2007. The survey covered demographic information, perceived health status, use of complementary therapy, cancer screening behavior, perceived susceptibility to cancer and family history of cancer. Descriptive statistics, percentages and logistic regression analysis were used for data analysis.Results:A total of 1,002 men aged 50 or above took part in the study (response rate=67%), and the uptake rate of PSA testing was found to be 10%. Employment status, use of complementary therapy, perceiving regular visits to a doctor as good for health and the recommendations of health professionals were signiifcant factors associated with PSA testing.Conclusion:The uptake rate of PSA testing in the study population was very low. Among all the factors identified, recommendations from health professionals had the strongest association with the uptake of PSA testing, and they should therefore take an active role in educating this population about cancer prevention and detection.

作者： 刊期： 2014年第01期