国际转化医学（英文版）杂志

作者： 刊期： 2013年第02期

Objective:To investigate the clinical value of total knee arthroplasty (TKA) on patients with advanced knee osteoarthritis. Methods:The clinical data and efficacy of 26 patients with advanced knee osteoarthritis (26 knees) who were given TKA in our department from June 2012 to May 2013 were retrospectively observed and analyzed. The knee function scores before operation and after follow up were evaluated according to American HSS scoring standard. Results:At the end of follow up, of the 26 patients, 18 were excellent, 6 were good and 2 were not bad in knee function and mobility without sense of pain, which was regarded to be associated with the poor enthusiasm in knee function training, and the total rate of excellent and good was 92.3%. Conclusion:TKA has signiifcant clinical value and favorable efifcacy on patients with advanced knee osteoarthritis.

作者： 刊期： 2014年第03期

Objective:To explore the effect of ibandronate on the proliferation and the expression of human telomerase reverse transcriptase (hTERT) of non-small cell lung cancer (NSCLC) A549 cell line in vitro. Methods: Methyl thiazolyl tetrazolium (MTT) assay, microscope, flow cytometry (FCM) and semi-quantitative RT-PCR were employed to detect the cell proliferation, cell cycle as well as the morphological change and the expression of hTERT mRNA of A549 cell line. Results:The data showed that ibandronate could effectively inhibit the proliferation of A549 cell line in time-and concentration-dependent. Under the microscope, the lfoating cells increased gradually as the drug concentration increasing. FCM detection showed that ibandronate could induce the cell cycle stopped in G0/G1 phase and downregulation expression of hTERT. Conclusion:Ibandronate can inhibit the proliferation of A549 cell line in vitro, whose mechanism may be associated with cell cycle arrestted in phase G0/G1 and downregulation expression of hTERT.

作者： 刊期： 2014年第03期

Cancer is always a main factor threatening human’s health and life, and its incidence and mortality are gradually increasing in recent years. However, some advances have been made with the unremitting efforts and exploration human made and the improvement is mainly made in cancer treatment of young children and older adults, while little in adolescent and young adult (AYA) patients, who are generally defined as individuals of 15 to 39 years old at the time of initial cancer diagnosis due to many factors. To highlight the issues of this unique population, National Comprehensive Cancer Network (NCCN) absorbs a large amount of information and previous researches and develops a set of clinical practice guidelines. Though the guidelines are more supportive care guidelines than treatment guidelines, they give us the opportunity to learn the latest international developments in AYA treatment and more survival chance for the treatment of AYA patients.

作者： 刊期： 2014年第03期

Objective:To explore the expression of ezrin and its signiifcance in non-small cell lung cancer (NSCLC). Methods:The expression of ezrin in 81 NSCLC patients were examined by immunohistochemical staining Envision method, and its relationships with the clinopathologic features and other indexes were analyzed. Results:The high expression rate of ezrin in NSCLC was 59.26% (48/81) and significantly correlated with lymph node metastasis (P < 0.05), but not associated with gender, histologic subtype, differentiation, TNM stages or smoking. The K-M survival analysis demonstrated that patients with the over-expression of ezrin were obviously poorer than those with low-expression of ezrin (P<0.05). Conclusion:The expression of ezrin may be associated with metastasis of NSCLC, can be as an important marker for evaluating the prognosis of NSCLC patients.

作者： 刊期： 2014年第03期

Background:Malignant spinal cord compression has signiifcantly increased hospitalization costs and even with best approach in treatment the disease course remains relatively stable with dire outcomes. Case presentation: The patient was an 80 years old male with the past medical history of hypertension, stroke with chronic right sided weakness, recently diagnosed with non-squamous cell lung carcinoma stage T4N0Mx presently undergoing chemotherapy as outpatient with carboplatin and taxol presented to the emergency room with the chief complaint of right leg pain with weakness and chest pain for 1~2 days. On d 4 of the admission patient complained of chest pain again and a CT angiogram was ordered as part of the work up for chest pain based on high probability for a pulmonary embolus per“Wells Score”. The CT angiogram revealed a large soft tissue mass centered at T5 vertebral body and probable spinal canal invasion. Conclusion:A more favorable outcome requires the input of both a surgeon and a radiation oncologist to ifnd the most effective approach depending on the area involved and the extent of the lesion, and patient’s choice of treatment always must be respected as well. Despite aggressive treatment patient did not respond well and was deteriorating. Options were discussed with the patient, including the futility of care and lack of response. Patient opted to return home with hospice care and was subsequently discharged home with family.

作者： 刊期： 2014年第03期

Most of cancer patients are suffering from psychological distress which is under-diagnosed and under-treated in China. The purpose of this study is to evaluate the psychometric properties of a new screening instrument for psychological distress in cancer patients based on Chinese culture and personality traits. The scale was created after face-to-face interviews and focus group discussion of 50 medical staffand 30 cancer patients; the analysis of reliability and validity came from 1122 scales completed by cancer patients and non-cancer patients. Data was analyzed for internal consistency reliability, construct validity and discrimination validity in clinical practice. The internal consistency reliability of the scale was 0.906. Principal components factor analysis and structural equation modeling showed that the scale was comprised of four dimensions: depression, anxiety, interpersonal barrier and suspiciousness. The ift indices were χ2(149)=257.594, χ2/d?=1.729, GFI=0.927, NFI=0.846, NNFI=0.906, RMSEA=0.061. These results indicated there were signiifcant differences between cancer patients and non-cancer patients, with respect to the total scores, anxiety scores, interpersonal barrier scores and suspiciousness scores. The psychological distress scale is a new screening instrument for Chinese cancer patients, which is in line with the emphasis of Chinese culture.

作者： 刊期： 2013年第02期

Objective: To investigate the effects of epileptic discharges in sleep of epileptic patients on sleep-breath events. Methods: Polysomnograph (PSG) and long-term video electroencephalogram (LTVEEG) were used to monitor 82 adult epileptic patients. The condition of paroxysmal events in nocturnal sleep was analyzed, and the epileptiform discharge and effects of antiepileptic drugs were explored. Results: In epileptic group, latency to persistent sleep (LPS) and REM sleep latency increased, the proportion of light sleep increased while that of deep sleep decreased, sleep efficiency reduced, nocturnal arousal times increased and apnea hyponea indexes (AHI) improved, which demonstrated significant differences by comparison to control group. Periodic leg movements (PLM) had no conspicuous differences compared with control group. There were no speciifc effects of epileptiform discharge and antiepileptic drugs on AHI and PLM indexes. Conclusion: Epileptic patients have sleep structure disorders and sleep-disordered breathing, and arousal, respiratory and leg movement events inlfuence mutually. Synchronous detection of PSG combined with LTVEEG is in favor of comprehensively analyzing the relationship between sleep structures and epilepsy-breath events.

作者： 刊期： 2013年第02期

作者： 刊期： 2014年第03期

Objective:To observe the expressions of 11beta-hydroxysteroid dehydrogenase types 1 and 2 in colorectal cancer. Methods:Neoplastic tissues and autologous non-neoplastic tissues were taken from 12 patients with colorectal cancer immediately after the operations. The mRNA expressions of 11beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) and 11beta-hydroxysteroid dehydrogenase type 2 (11β-HSD2) were detected by reverse transcription polymerase chain reaction (RT-PCR). The protein expressions of 11β-HSD1 and 11β-HSD2 were detected by Western-blot assay. Results:Both isoforms of 11β-HSD expressed in the colon adenocarcinoma, but their expressions were not identical in neoplastic and non-neoplastic tissues. There was a significant decrease of 11β-HSD2 mRNA abundance and protein abundance in neoplastic tissue. 11β-HSD1 protein and mRNA abundance were not signiifcantly different from that in control tissue. Conclusions:Neoplastic transformation is associated with the decreases of 11β-HSD2 mRNA and protein.

作者： 刊期： 2014年第03期