国际转化医学（英文版）杂志

Objective:To explore the effect of ibandronate on the proliferation and the expression of human telomerase reverse transcriptase (hTERT) of non-small cell lung cancer (NSCLC) A549 cell line in vitro. Methods: Methyl thiazolyl tetrazolium (MTT) assay, microscope, flow cytometry (FCM) and semi-quantitative RT-PCR were employed to detect the cell proliferation, cell cycle as well as the morphological change and the expression of hTERT mRNA of A549 cell line. Results:The data showed that ibandronate could effectively inhibit the proliferation of A549 cell line in time-and concentration-dependent. Under the microscope, the lfoating cells increased gradually as the drug concentration increasing. FCM detection showed that ibandronate could induce the cell cycle stopped in G0/G1 phase and downregulation expression of hTERT. Conclusion:Ibandronate can inhibit the proliferation of A549 cell line in vitro, whose mechanism may be associated with cell cycle arrestted in phase G0/G1 and downregulation expression of hTERT.

作者： 刊期： 2014年第03期

Objective:To comprehend the characteristics of patients with positive human immunodeifciency virus (HIV) (1+2) antibodies in the preliminary screening so as to provide basis for local HIV screening and prevention. Methods:Enzyme-linked immunosorbent assay (ELISA) was used to detect serum HIV (1 +2) antibodies in the preliminary screening, after which the positive serum was sent to acquired immune deficiency syndrome (AIDS) confirmatory laboratory to be confirmed with western blotting method. Clinical data of patients with positive HIV antibodies in the preliminary screening in the outpatients and inpatients from 2006 to 2013 were collected and analyzed. Results:A total of 394 patients with positive serum HIV antibodies were screened initially, in which 214 were confirmed positive HIV, 13 were not certain and another 167 were negative. Patients with positive serum HIV antibodies in the preliminary screening were increased from 9 cases in 2006 to 94 cases in 2013, in which those conifrmed with positive HIV increased from 5 to 49. Patients with positive serum HIV antibodies in the preliminary screening and those conifrmed increased annually. In addition, patients confirmed with positive serum HIV antibodies were mainly males and aged 20 ~ 49 years, distributing in Departments of Infections, Respiratory, Dermatology, Hematology and Emergency, whereas those confirmed with negative HIV were mainly females and aged >20 years, distributing in Departments of Hematology, Maternity and Emergency as well as Reproductive Center. Conclusion: HIV infection is in low level with characteristics of annually increasing infection rate, male-orientated and wide-spread distribution in variuos departments, etc., knowing which will help guide the clinical practices and carry out the local HIV screening and prevention by relevant departments.

作者： 刊期： 2014年第03期

As a new branch of medicine with unlimited prospect, Translational Medicine has been rapidly developed over the past decades and gained global attention. Translational Medicine can be vividly described as“bench to bedside (B2B)”model which focuses on the integration of multiple aspects, especially the basic medicine and clinical medicine, and has a great potential in treating many severe diseases and improving human health. Medical journals are carriers for medical information and play a great role in the development of medicines, and Journal of International Translational Medicine (JITM) is just a journal emerging at the right moment and specialized in translational medicine. With professionalized attitude and internationalized requirements, JITM is committed to promote the development of Translational Medicine.

作者： 刊期： 2014年第03期

A case of a 53-year-old man with adult T-lymphoblastic lymphoma (T-LBL) in mediastinum who survived more than 33 months in our hospital was reported. LBL was more common in school-age and teenage boys than adults. The reported patient manifested progressive dyspnea. Chest CT scanning showed a huge irregular soft tissue shadow in anterior mediastinum which extended into posterior mediastinum and left chest wall. The tumor was 20 cm×15 cm×15 cm in size in left upper mediastinum and its boundaries with pericardium and left lower lung were unclear. The diagnosis of T-lymphoblastic lymphoma was confirmed by immunohistochemical staining. After surgery, he received five chemotherapy courses including Gem, CTX and VCR and biological therapies with IL-2 and IFN-α2b. About 16 months later, he complained of diplopia in the left eye without any evidence of recurrence. Blood examination which showed leukemoid reaction twice returned to normal after dehydration, hormone and thalidomide treatments. Finally, his condition became worse and died of pulmonary infection 33 months after ifrst medical service. In summary, T-LBL is a rare disease which may result in poor prognosis even if the patient has received immediate chemotherapy. Hormone and thalidomide can be applied to treat adult T-LBL.

作者： 刊期： 2013年第02期

Objective:To explore the proliferation inhibition and apoptosis-inducing effect of evodiamine in human gastric cancer SGC-7901 cells. Methods:After 48 or 24 h exposure to different concentrations of evodiamine, cell proliferation was analyzed using tetrazolium blue (MTT) assay while apoptosis and cell-cycle phase distribution using lfow cytometry. Results:In 0.01~30.00μg/mL range of concentrations, evodiamine inhibited the proliferation of SGC-7901 cells in dose-dependent manner, and the overall mean IC50 was (3.79±0.16)μg/mL;the apoptosis rate was increased from 3.4%to 7.0%, 13.8%and 36.3%at concentrations of 0, 0.5, 1.5 and 30μg/mL of evodiamine, respectively;the percentage of cells accumulated in G2/M phase was increased from 17.26%to 98.92%in the cells treated with evodiamine for 24 h in 0.01~30.00μg/mL range of concentrations. Conclusion:Evodiamine can inhibit the proliferation, induce apoptosis in human gastric cancer cell line SGC-7901 in vitro and arrest the cell cycle at the G2/M phase.

作者： 刊期： 2014年第03期

Objective:To investigate the clinical value of total knee arthroplasty (TKA) on patients with advanced knee osteoarthritis. Methods:The clinical data and efficacy of 26 patients with advanced knee osteoarthritis (26 knees) who were given TKA in our department from June 2012 to May 2013 were retrospectively observed and analyzed. The knee function scores before operation and after follow up were evaluated according to American HSS scoring standard. Results:At the end of follow up, of the 26 patients, 18 were excellent, 6 were good and 2 were not bad in knee function and mobility without sense of pain, which was regarded to be associated with the poor enthusiasm in knee function training, and the total rate of excellent and good was 92.3%. Conclusion:TKA has signiifcant clinical value and favorable efifcacy on patients with advanced knee osteoarthritis.

作者： 刊期： 2014年第03期

Ebola virus disease (EVD) is an acute hemorrhagic infectious disease caused by ebola virus, with high infectivity and fatality rate. At present, it mainly occurs in areas of Central Africa and West Africa and no effective vaccine and antiviral drugs are available for the clinical treatment.

作者： 刊期： 2014年第03期

作者： 刊期： 2013年第02期

Objective:To observe the expressions of 11beta-hydroxysteroid dehydrogenase types 1 and 2 in colorectal cancer. Methods:Neoplastic tissues and autologous non-neoplastic tissues were taken from 12 patients with colorectal cancer immediately after the operations. The mRNA expressions of 11beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) and 11beta-hydroxysteroid dehydrogenase type 2 (11β-HSD2) were detected by reverse transcription polymerase chain reaction (RT-PCR). The protein expressions of 11β-HSD1 and 11β-HSD2 were detected by Western-blot assay. Results:Both isoforms of 11β-HSD expressed in the colon adenocarcinoma, but their expressions were not identical in neoplastic and non-neoplastic tissues. There was a significant decrease of 11β-HSD2 mRNA abundance and protein abundance in neoplastic tissue. 11β-HSD1 protein and mRNA abundance were not signiifcantly different from that in control tissue. Conclusions:Neoplastic transformation is associated with the decreases of 11β-HSD2 mRNA and protein.

作者： 刊期： 2014年第03期

Cancer is always a main factor threatening human’s health and life, and its incidence and mortality are gradually increasing in recent years. However, some advances have been made with the unremitting efforts and exploration human made and the improvement is mainly made in cancer treatment of young children and older adults, while little in adolescent and young adult (AYA) patients, who are generally defined as individuals of 15 to 39 years old at the time of initial cancer diagnosis due to many factors. To highlight the issues of this unique population, National Comprehensive Cancer Network (NCCN) absorbs a large amount of information and previous researches and develops a set of clinical practice guidelines. Though the guidelines are more supportive care guidelines than treatment guidelines, they give us the opportunity to learn the latest international developments in AYA treatment and more survival chance for the treatment of AYA patients.

作者： 刊期： 2014年第03期