正常皮肤和瘢痕组织表皮干细胞定位与增殖分化特征的比较性研究

A型肉毒毒素治疗痉挛性斜颈14例

痉挛性斜颈是临床上为常见的局灶性肌张力障碍性疾病,为头颈部肌肉不协调过度收缩,而致一些转头动作或持续性异常头位,可伴痉挛性痛.作者在1999年12月～2002年8月应用A型肉毒毒素局部注射治疗痉挛性斜颈患者14例,取得了满意的临床效果,现报告如下.

作者：曲芳；胡建华；刘英 刊期： 2002年第24期

下颌前部水平截骨颏成形术对患者术后感觉障碍影响的研究进展

This article summarized exposure area, treatment of mental nerve, soft tissue stalk, osteotomy position and fixation method. 1 Mental part exposure area and treatment of mental nerve.

作者：刘妍琼；华泽权 刊期： 2002年第24期

牵引并手法治疗混合型颈椎病70例

Background:Traction of cervical vertebrae is an effective method in treatment of cervical spondylopathy. Manual therapy can correct dysfunction,has the effects of function exercises and has an active significance to the rehabilitation of mixed cervical spondylopathy.Combination of these two methods could enhance therapeutic effects and do good to functional recovery.

作者：李兴海；李丹 刊期： 2002年第24期

血管内皮细胞与聚羟基丁酯细胞相容性的实验研究

目的评价新型可降解生物材料聚羟基丁酯(PHB)与血管内皮细胞的细胞相容性.方法采用培养的牛血管内皮细胞接种在PHB膜片及胶原包埋的PHB膜片上,并与PGLA和空白对照作比较,用相差显微镜和扫描电镜观察细胞的粘附和生长情况,并对细胞增殖用MTT法进行测定.结果牛血管内皮细胞在四个实验组中粘附良好,生长符合其生长曲线,胶原包埋PHB较单纯PHB细胞更易粘附生长,较PGLA略差.结论牛血管内皮细胞与PHB细胞相容性好,其用胶原包埋可增加细胞亲和性.

作者：袁文周；艾玉峰；熊猛；夏文森；张琳西；董兆麟；杨清芳 刊期： 2002年第24期

肘管综合征15例肌电图特征分析

尺神经在肘部损害或长时间肘关节伸直固定,从事某种职业而产生的职业性迟发性尺神经麻痹的症状体征.患者手步感觉和运动障碍,严重的手功能受损.因此早期诊断及时治疗是十分重要的.肌电图检查对于该病的诊断及治疗结果判定均有重要的价值.该病对15例肘管综和征进行诊断分析.

作者：陈慧娟；王桂风；高敏 刊期： 2002年第24期

脊髓横断损伤后局部灌注地塞米松对中枢神经系统胶质细胞增殖与神经细胞轴突的影响

目的研究局部使用类固醇激素对损伤后中枢神经系统胶质细胞增殖和神经轴突变性坏死的影响.方法成年Wistar大鼠18只全部切断T10脊髓,6只横断间隙灌注地塞米松(DXM)为实验组,6只腹腔内注射DXM为全身用药组,另6只脊髓横断间隙灌注生理盐水为对照组.存活鼠5d后灌流固定取材石腊切片.选用神经中丝抗体(antineurofiblamentNF)和胶质纤维酸性蛋白抗体(antiglialfibriallaryacidicproteinGFAP)对损伤脊髓的轴突和胶质细胞进行标记,用图象分析系统分析实验结果并进行分组统计学处理.结果实验组在损伤的脊髓残端GFAP阳性表达下调,而NF阳性表达上调,对照组结果相反,阳性面积均值实验组与对照组相比差异有显著性意义(P<0.01);实验组、腹腔注射组NF阳性表达均上调且二者出现差异有显著性意义(P<0.01);实验组、腹腔注射组GFAP表达差异有显著性意义,腹腔注射组与空白对照组GFAP表达结果比较差异也有显著性意义.结论脊髓损伤局部灌注与全身使用DXM相比可以有效地抑制胶质细胞增殖、保护神经细胞损伤后的变性坏死.

作者：胡大勇；翁羽中；胡兵；周逸峰 刊期： 2002年第24期

颅骨修补术后局部疼痛分析1例报告

1 GENERAL DATA Case condition:Famle,aged 35 years old,craninal bone repair was performed on May 1998.deficiency of cranial 5 cm× 5 cm,region:tempora and parietal area.Operative time:type of operation is II type repair,repair material was fixed with double size 7 silk,and moulded under local intensified anesthesin.Wound were duched with gentamycin sulfate liquid,drain was laid,removed on next day.Secondary operation:Result of exploration(leading to pain):subcutaneous hydrops.

作者：黎铁伟 刊期： 2002年第24期

血管内治疗老年动脉瘤降低致残率的随访分析

目的探讨老年动脉瘤电解可脱性弹簧圈血管内栓塞治疗颅内动脉瘤技术要点及降低致残率效果.方法对34例老年动脉瘤应用微导管技术,在数字减影血管造影监视下行血管内栓塞治疗,采用电解式可脱性弹簧圈(GDC)栓塞.2例巨大动脉瘤用可脱性球囊闭塞载瘤动脉.结果成功栓塞34例36个动脉瘤,32例痊愈,1例需人照顾,1例重残,明显降低致残率.栓塞程度:100%栓塞28个(包括载瘤动脉闭塞者),95%栓塞3个,90%栓塞3个,80%栓塞2个.结论老年动脉瘤患者常合并心、肺、肾等其他器官疾病及高血压、高脂血症、糖尿病等并发症,脑血管硬化、迂曲明显,但血管内栓塞治疗是一种比较安全、可靠、有效的治疗手段,可明显降低患者残疾程度.

作者：梁国标；魏学忠；李智勇；杜鹏；韩松；唐新华 刊期： 2002年第24期

跟骨距下关节内移位骨折的手术治疗与术后康复

Background:Fracture of calcaneus often leads to pain,deformity,function of foot is impaired and even permanent function impairments are left.Many divergence existed in treatment of severe intra articular fracture existed,but with the development of pedal surgery and deep study of biomechanics anatomy,especially building of BO theory,the therapeutic programs have become clearer.

作者：金晨；胡小鹏；杨裕红；张德常；陈学桂；吴揭地 刊期： 2002年第24期

肘管综合征的肌电图意义

电生理检查可为临床提供肘管综合征可靠的辅助诊断依据,并为手术提供较准确的嵌压部位.将1997年以来收治的33例患者及6例手术证实的该病例报告如下.

作者：郑晓君；李军；王苏平 刊期： 2002年第24期

大鼠实验性脑震荡脑组织中NSE和GFAP表达研究

Objective To study the changes and significance of neuron specific enolase(NSE) and glial fibrilous acidic protein(GFAP) in rat cerebral concussion.Methods 80 Wistar male rats were used for animal model of cerebral concussion,which were sacrificed on the 1st,3rd, 7th,14th and 30th days after injury and the brain tissue were taken off.The expressions of NSE and GFAP were studied in the course of cerebral concussion by means of immunohistochemistry. Results Rats in 100 g group were seen the clinical manifestation for typical concussion.The pathologic changes were the cerebral vascular constriction and dilation,congestion and edema of cerebral tissue and neuronal degeneration and necrosis.NSE was increased on the 1st day,and the positive area was seen in the plasma of the neurons in the cerebral cortex and the cerebellum,and also seen in blood vessels,cerebrospinal fluid in aqueduct and interstitial matrix.NSE was obtained at peak on the 7th day,decreased on the 14th day and still raised on the 30th day.GFAP was increased on the 1st day,which the positive area was seen in the plasma of astrocytes,and obtained at peak on the 3rd day,which fiber like GFAP was in short,thick and astrocytes increased.GFAP decreased on the 7th day and obtained normal level in 30 days.Conclusion The main pathologic changes of cerebral concussion were blood circulatory disorder and nervous cells degeneration,apoptosis and necrosis.NSE and GFAP participated in the course of cerebral concussion,may play an important role in the damage of blood brain barrier, nervous cells degeneration and necrosis.

作者：彭瑞云；高亚兵；王德文；肖兴义；陈浩宇；吴小红；刘杰；胡文华；蔡宝仁；薛官生；张援平；尹晓梅 刊期： 2002年第24期

不同年龄增生性瘢痕转化生长因子β1表达的检测及其意义

目的通过观察不同年龄增生性瘢痕与正常皮肤组织中转化生长因子β1(TGFβ1)的表达,初步探讨TGFβ1的表达与增生性瘢痕形成的年龄因素的关系.方法以增生性瘢痕为研究对象,正常皮肤组织为对照,采用SABC免疫组化方法观察1～19岁、20～50岁增生性瘢痕与各年龄组正常皮肤的细胞因子TGFβ1的表达.结果增生性瘢痕组织的TGFβ1含量显著大于正常皮肤.1～19岁增生性瘢痕组TGFβ1表达较20～50岁增生性瘢痕组的增高差异有具显著性意义(P<0.01).胎儿组皮肤TGFβ1表达低于正常皮肤(P<0.01).结论TGFβ1表达增高是增生性瘢痕形成的原因之一.

作者：邱林；金先庆；向代理；付跃先；田晓菲 刊期： 2002年第24期

运动训练对热损伤患儿肺功能的影响

作者： 刊期： 2002年第24期

针灸结合中药治疗跟痛症18例

Background:Calcaneodynia refers to pain caused by traumatic injuries and is often combined with spur of front edge of calcaneus.Although there are many therapeutic methods to this disease,effects aren't satisfying.

作者：朱静芬 刊期： 2002年第24期

人重组骨形成蛋白-2对骨髓成骨细胞增殖和碱性磷酸酶活性的影响

目的探讨人重组骨形成蛋白-2(rhBMP2)对人骨髓成骨细胞增殖和分化的影响以及剂量效应关系.方法以改良方法培养的骨髓成骨细胞为作用底物,加入梯度浓度的rhBMP2溶液,应用MTT法和碱性磷酸酶(ALP)染色法检测其对细胞增殖和ALP活性的影响.结果rhBMP2浓度低于1μg/ml时对人骨髓成骨细胞的增殖无明显影响,高于此浓度时对细胞的增殖有明显的促进作用,但无显著的剂量效应关系.从0.1～10μg/ml,ALP与rhBMP2具有剂量依赖性促进关系,rhBMP2浓度为10μg/ml时,其ALP活性是对照组的4倍,在高于10μg/ml时ALP活性并没有进一步显著提高.结论rhBMP2对骨髓成骨细胞的增殖和碱性磷酸酶活性具有促进作用,其佳效应浓度为10μg/ml.

作者：卢丙仑；冯志华；刘宝林；洪咏龙；汤苏阳 刊期： 2002年第24期

低功率毫米波对肿瘤患者红细胞、淋巴细胞免疫粘附功能的影响及相互作用

Objective To study the effects of low intensity millimeter wave(MMW) irradiation on the immune adhesion function of erythrocytes and lymphocytes in tumor patients.Methods MMW(36 GHz,0.73- 1.46 mW/cm2) was used to irradiate the vein blood from tumor patients in irradiation group for 30 minutes.Control group received false irradiation using the same method.Then test tumor RBC C3b receptor rosettes rate(RCR),tumor RBC rosette rate(TRR) and tumor lmphocyte rosette rate(TLR).Result In irradiation group,the RBC C3b TRR and TLR were higher than control group's(P< 0.01).Conclusion Low intensity MMW irradiation can improve the immune adhesion function of erythrocytes and lymphocytes in tumor patients.

作者：李雪平；陈景藻；杨红；郭峰 刊期： 2002年第24期

苯经呼吸道吸入对大鼠胚胎的致畸毒性

目的探讨经呼吸道吸入苯,对大鼠胚胎是否有致畸作用及其剂量效应.方法孕鼠随机分组后采用静式吸入染毒柜(500L),于妊娠第5～16天(胎鼠器官形成期)每剂量组分别每日以不同浓度染毒1次,90min/次,妊娠第20天处死母鼠,剖腹观察对胚胎的影响程度.结果经呼吸道吸入一定量的苯(浓度15mg/m3,90min/次,1次/d)对大鼠胚胎发育有明显影响,与对照组比较差异有显著性意义.结论苯经呼吸道吸入对大鼠胚胎有致畸作用.

作者：刘钧；赵玉元；高明太；赵成基 刊期： 2002年第24期

运动训练与传统的门诊治疗对严重烧伤患儿康复效果比较

作者： 刊期： 2002年第24期

颅内电极监测癫痫发作初始期脑电定位意义

目的探讨颅内电极监测癫痫发作初始期脑电对致痫灶的定位价值.方法14例难治性癫痫患者,其临床、影像学检查及头皮脑电记录等不能精确定位致痫灶,经颅骨钻孔、埋置颅内深部和/或皮层电极,长程脑电监测并分析发作初始期脑电的异常放电节律和范围,对比术后病理学检查和随访结果,分析发作初期脑电定位致痫灶的准确性.结果记录到了47次临床发作.发作初期异常放电可分为4种形式:低幅快节律、尖波节律、高幅棘波节律和棘慢波节律.按异常放电范围可分为:局限性放电、区域性放电和广泛性放电.11例(78.5%)患者准确定位了致痫灶,术后癫痫发作消失(64.3%)或极少发作(14.2%),2例(14.2%)发作减少90%以上,1例(7.1%)术后无改善.结论颅内埋置电极脑电监测癫痫发作初始期异常放电形式、部位和异常放电范围是定位致痫灶可靠的方法.

作者：张国君；王玉平；遇涛；李勇杰 刊期： 2002年第24期

人工髋关节置换术后的功能锻炼

Background: The effects that total hip replacement (THR) can alleviate pain, reconstruct function of hip joint and improve patients' daily living ability have been proved, success has a direct relationship with postoperative function exercises.

作者：靳徐凤 刊期： 2002年第24期