刘晓阳；汤继文
Objective To construct an eukaryote expression vector containing bone morphogenetic protein 2 (BMP 2) mature peptide. Methods Gene splicing by overlapping extension PCR (SOE PCR) method was used to clone BMP2 signal peptide and mature peptide and their fusion fragment.The fusion fragment was cloned into an eukaryote expressing vector pcDNA3.1/myc His(- )A. The sequence of the fusion fragment of BMP2 signal peptide and mature peptide was identified.Results The sequence of the fusion fragment was correct comparing with BMP2 signal peptide and mature peptide published by NCBI.Conclusion The vector pcDNA3.1/myc His(- )A BMP2sm constructed in this experiment was suitable to applying in eukaryotic expression of BMP2.
作者：段小红；陈苏民；柴玉波；徐可为 刊期： 2002年第24期
作者： 刊期： 2002年第24期
Most of patients with intertrochanteric fracture of femur were treated by operations, such as Duogensi needle,Jewett pin,AO angle steel plate, Gamma pin and Richards pin. We treated 28 cases of intertrochanteric fracture of the femur with dynamic condylar screw from January 1997 to January 2000. Report as follow:
作者：洪加源；康两期；郭林新；练克俭；丁真齐；郭延杰；翟文亮；郭志民 刊期： 2002年第24期
Background:The effect of drugs therapy on scapulohumeral periarthritis is not obvious and medical gymnastics is an effective method for preventing and curing some patients.
作者：王大平；窦文强；周祖保；喻祝仙 刊期： 2002年第24期
目的文章探讨关节突关节形态对腰椎滑脱的影响.方法L4～5节段退变性滑脱25例(均年龄55岁)、后滑脱12例(均年龄53岁)和峡部裂滑脱11例(均年龄44岁)与32例(均年龄47岁)正常人进行对照检查.CT轴向扫描关节突关节,测量L3～4、L4～5、L5～S1关节突关节角度、关节宽度、关节面的深度和关节面形态.结果由上向下关节角度逐步从矢状向冠状转化.在L4～5节段退变性滑脱患者关节角度方向与对照组相比明显偏向矢状(P<0.01),退变性滑脱组和后滑脱组的关节宽度均大于对照组(P<0.01),退变性滑脱组的关节面深度比也有显著差异.峡部裂滑脱组的唯一差异是关节宽度显著减少(P<0.01).结论结果提示关节突关节矢状排列可能是生长过程中形成的,某些患者发生腰椎退变时可能会出现滑脱.
作者：杨卫新；章稼；何怀；昝云强 刊期： 2002年第24期
应用神经导管的依据是将导管套接于缺损神经的两端,神经再生即可通过管腔来进行,可以预防神经间隙内神经瘤形成和纤维组织进入,更重要的是,远侧神经支产生的趋化性和营养性因子能在导管内积聚,营养性因子有助于损伤轴突的存活和生长,趋化性因子则对再生轴突的生长方向有重要影响[1-2].
作者：王相如；钱济先 刊期： 2002年第24期
目的探讨截肢后残肢功能重建的综合康复治疗效果.方法通过22例截肢者功能训练、肢体理疗、按摩、中药外敷及再手术原因进行分析探讨.结果22例截肢患者通过综合康复指导治疗,使残端愈合时间,残端定型,关节功能,穿用假肢后肢体功能等诸方面均有明显提高.结论采用综合康复治疗,使残肢残而不废,尽量发挥大功能.
作者：韩作峰；祁秀 刊期： 2002年第24期
Objective To study the effect of total body irradiation on the quantity and activity of P65,TNF α and IL 1 in irradiated rat wounds. Methods Tumor necrosis factor α (TNFα ) and interleukin 1 (IL 1 ) in wound fluids were quantified by ELISA and their biological activities were measured by L929 cell lysis and thymocyte proliferation,respectively. The expression of P65 protein in wounded tissue was determined by western blotting. Results The biological activities of TNF α and IL 1 in wound fluid decreased significantly after irradiation,while their quantities in wound fluids did not decrease significantly.The expression of P65 protein in wounded tissue also did not decrease obviously after irradiation. Conclusion These results suggestted that total body irradiation may inhibit the biological activities of TNF α , IL 1 and nuclear factor B,there may exist negative factors to disturb the protein functions in wound environments.
作者：朱颖；史春梦；程天民 刊期： 2002年第24期
Background:Fracture of calcaneus often leads to pain,deformity,function of foot is impaired and even permanent function impairments are left.Many divergence existed in treatment of severe intra articular fracture existed,but with the development of pedal surgery and deep study of biomechanics anatomy,especially building of BO theory,the therapeutic programs have become clearer.
作者：金晨；胡小鹏；杨裕红；张德常；陈学桂；吴揭地 刊期： 2002年第24期
作者： 刊期： 2002年第24期
目的研究局部使用类固醇激素对损伤后中枢神经系统胶质细胞增殖和神经轴突变性坏死的影响.方法成年Wistar大鼠18只全部切断T10脊髓,6只横断间隙灌注地塞米松(DXM)为实验组,6只腹腔内注射DXM为全身用药组,另6只脊髓横断间隙灌注生理盐水为对照组.存活鼠5d后灌流固定取材石腊切片.选用神经中丝抗体(antineurofiblamentNF)和胶质纤维酸性蛋白抗体(antiglialfibriallaryacidicproteinGFAP)对损伤脊髓的轴突和胶质细胞进行标记,用图象分析系统分析实验结果并进行分组统计学处理.结果实验组在损伤的脊髓残端GFAP阳性表达下调,而NF阳性表达上调,对照组结果相反,阳性面积均值实验组与对照组相比差异有显著性意义(P<0.01);实验组、腹腔注射组NF阳性表达均上调且二者出现差异有显著性意义(P<0.01);实验组、腹腔注射组GFAP表达差异有显著性意义,腹腔注射组与空白对照组GFAP表达结果比较差异也有显著性意义.结论脊髓损伤局部灌注与全身使用DXM相比可以有效地抑制胶质细胞增殖、保护神经细胞损伤后的变性坏死.
作者：胡大勇；翁羽中；胡兵；周逸峰 刊期： 2002年第24期
Background: The common mood reaction in breast cancer was depression and mental test by Zung's self evaluated depression scale showed that different degrees of depression existed in 100% patients, so, mental nursing was necessary.
作者：段艳萍；冯莉娟；丁小蓉；杜燕 刊期： 2002年第24期
腰三横突综合征是康复科临床的常见病之一,其发病率高,多见于青壮年体力劳动者.近年来,采用龙虎交战刺法治疗腰三横突综合征169例,并与对照组52例比较,现将结果总结如下.
作者：兰春燕；孙德芝；王秀珍 刊期： 2002年第24期
腰椎间盘突出症是指椎间盘发生退行性改变,其中50%～85%的病例可引起坐骨神经痛,其疼痛性质为典型的根性神经痛.中国长城铝业公司总医院康复科应用非手术康复治疗31例急性疼痛的重症住院患者,疗效显著.
作者：潘守政；张磊；陈红 刊期： 2002年第24期
响针刺法辅以点穴治疗软组织损伤引起急性疼痛,针刺时患者唯闻响音又不出现痛麻胀沉等显性针感,却有针响痛退之奇效.从1995年开始,采用该法治疗软组织损伤急性疼痛,并与常规刺法进行对比观察,现整理资料报道如下.
作者：李富平；杨贵清；李婧 刊期： 2002年第24期
Objective To observe influence of fibrin sealant(FS) on osteoinductive ability of inject type BMP.Method The inject type BMP power was dissolved in the main glue part or thrombin part of FS, then mixed with the main glue part or thrombin part of FS into gel, observe coagulating time, then implant composite into the thigh muscle pouch of mice to evaluate their capacity to induce new bone formation, and compared to the single BMP implant group.Result There was no difference in the coagulating time between two mixing method, the osteoinductive ability of implants BMP dissolved in the main glue part or thrombin part of FS group was higher than that of simply BMP implant group.Conclusion FS was perfect carrier to inject type BMP.
作者：王登虎；刘建；李丹；胡蕴玉；袁志 刊期： 2002年第24期
假肢装配是个非常复杂的人-机-环境相结合的过程.截肢者需要终生使用假肢.假肢装配后,穿戴在截肢者身上进行反复的适合检查、修改、调整是保证假肢装配质量,尽量减少长期使用假肢引起并发症的重要方法.假肢适合检查分为初检、终检两个阶段.初检的假肢是初步组装的半成品,便于修改、调整,应当反复检查,反复修改.终检的假肢是已完成的产品.文章介绍了小腿假肢、大腿假肢适合检查的主要内容,检查方法、基本要求和常见问题.
作者：赵辉三 刊期： 2002年第24期
Background: According to small scale of investigation, world health organization (WHO) infers that moderate and serious pain is observed in about 1/3 of patients receiving active anti cancer therapy and 60% in late stage. Cancer related pain is a threat to patients' living quality and confidence, so rehabilitation becomes an important part in treatment of cancer.
作者：蔡欣；孙亮新；张洁；周涛；宋秀珍；刘基巍 刊期： 2002年第24期
目的进行β-磷酸三钙/聚乳酸叠层复合支架的应用评价.方法在体外37℃生理盐水中降解过程中,观察该材料的重量、力学强度、聚乳酸分子量、材料周围环境的pH值、Ca2+浓度等参数随时间的变化情况,以及将该支架材料和骨髓基质细胞复合后,植入中国青山羊胫骨段缺损处进行修复实验.结果对修复效果进行了观察,发现其成骨能力较强.结论此种材料在骨组织工程领域有一定应用前景.
作者：汤顺清；石海涛；毛萱；周长忍；裴国献；陈滨 刊期： 2002年第24期
目的探讨三维建模及有限元分析对肱骨骨折治疗的临床意义,为肱骨骨折治疗轴向加载提供一定理论依据.方法以肱骨横截面CT图像为基础,建立肱骨模型,利用ANSYS5.6软件进行计算,选取三维十结点四面体单元,共划分2729个结点、49041个单元,分析骨折断面与轴线成30°,45°,90°及X、Y、Z3方向固定时肱骨轴向受压的应力分布及大小.结果肱骨断面与非断面处应力差异较大,断面处应力较集中,且以断面为中心呈非严格对称分布,应力较大区域分布在距骨折部位10mm左右,是其它应力区的两三倍.结论肱骨骨折治疗中应视不同情况估算所需载荷大小以选择合适内固定植入物.
作者：陈宗和；苏佳灿；张春才；许硕贵；田甜；张雪松；薛召军；吴建国；丁祖泉 刊期： 2002年第24期
中国组织工程研究杂志
主管:中华人民共和国卫生部
主办：中国康复医学会,《中国组织工程研究与临床康复》杂志社
