Objective:The purpose of the study is to investigate the ef ects of up-regulation of Raf kinase inhibitor protein (RKlP) on the chemosensitivity of cervical cancer Hela cells. Methods:Eukaryotic expression plasmid pcDNA3.1(+)-ssRKIP containing human overal length RKIPcDNA was transfected into cervical cancer Hela cellby lipofectin assay, establishing a stable cellline containing a target gene by G418. Expression of RKIP in Hela cells was measured by Western blot analysis. After treatment with cisplatin of dif erent concentrations and intervals of time, the ef ect of RKIP on the proliferation of Hela cells was evaluated by MTT method. The flow cytometry was used to investigate whether the RKIP could inhibit apoptosis in Hela cells induced by cisplatin. Results:The expression of RKIP in Hela cells transfected with pcDNA3.1-ssRKIP was increased obviously. After dif erent concentrations of cisplatin treatment cells for 24, 48 and 72 h, the growth inhibition rate in Hela cells transfected with pcDNA3.1-ssRKIP was significantly higher than in control cells (P<0.05). With 5μg/mL cisplatin treatment for 24 h, pcDNA3.1-ssRKIP-transfected Hela cells had an obviously higher percentage of apoptosis (23.2 ± 0.24)%than non-transfected cells (12.4 ± 0.31)%and empty vector-transfected cells (13.4 ± 0.47)%. Without treatment of cisplatin, the percentage of apoptosis for Hela cells transfected with pcDNA3.1-ssRKIP was (5.7 ± 0.12)%, which was stil higher than those of the non-transfected cells (2.9 ± 0.21)%and empty vector-transfected cells (3 ± 0.08)%. Conclusion:Higher expres-sion of RKIP gene can improve chemosensitivitv of cervical cancer Hela cells to cisplatin.
作者： 刊期： 2014年第03期
Castration-resistant prostate cancer (CRPC) is defined as prostate cancer that recurs while a patient is receiving androgen deprivation therapy (ADT). Many treatment options have been suggested for this chal enging disease;starting from 2-year hormonal manipulations, mitoxantrne-and docetaxel-based regimens reaching to the overwhelming new systemic op-tions for CRPC (newer hormonal treatments, cytotoxic chemotherapies, bone-targeted agents and immunotherapeutics);and the question is:do the traditional cytotoxic regimens stil have a role amidst al these new options?
作者： 刊期： 2014年第03期
Objective:The aim of our study was to investigate the ef ects of the anti-tumor composition of the acetoacetate extract of Vitex Negundo Seed (EVn-50) on the growth of human cervical carcinoma HeLa cells xenografts in nude mice and its possible molecular mechanism. Methods:Models of human cervical cancer HeLa cells xenografts transplanted subcuta-neously in nude mice were established and randomly divided into 7 groups (each group including 5 nude mice):saline group, Taxol group, EVn-50 group, comp-6 group, comp-7 group, comp-8 group and comp-10 group. The volume and weight of Xe-nograts were observed and compared. The alteration of the weight of nude mice, and the change of serum levels of LDH, ALT, Cr and WBC counts were examined and compared. The apoptotic rate of human cervical carcinoma HeLa cells xenografts was analyzed by FCM. The expressions of P53 and Bcl-2 proteins of HeLa cells xenografts were determined by Western blot-ting. Results:EVn-50 and its fractionated extracts could significantly suppress the increasing volume and weight of human cervical carcinoma HeLa cells xenografts in nude mice models in time-dependent manner, yet had no significant ef ect on the weight of nude mice, the serum levels of LDH, ALT, Cr and WBC were counted. When the xenografts were treated with EVn-50 and its fractionated extracts for 16 days, the apoptotic rate of xenografts cells were significantly increased, and the expression of P53 protein was up-regulated and protein level of Bcl-2 was decreased. Conclusion:EVn-50 and its fractionated extracts could suppress the growth of human cervical carcinoma HeLa cells xenografts in nude mice, which may be related to its pro-motion on xenografts cells apoptosis through down-regulation of Bcl-2 expression and activation of P53 expression.
作者： 刊期： 2014年第03期
Objective:The aim of this study was to explore the diagnostic value of multi-slice computer tomography (MSCT) in solitary pulmonary nodule (SPN). Methods:Contrastive analysis of the dif erences of CT signs between benign and malig-nant SPN. Results:The typical sign of pleural indentation was a reliable sign for lung cancers. Vacuole sign suggested lung cancer highly. Blood vessels cluster involving the vein alone had big opportunity to lung cancer. Type I tumor-bronchial relation was the most common relation in lung cancers. Type V tumor-bronchial relation was the most common relation in benign SPN. Conclusion:MSCT had a very high diagnostic value in SPN.
作者： 刊期： 2014年第03期
Objective:The aim of our study was to observe the survival and morphological changes of thawed ovarian tis-sues after heterotopic transplantation. Methods:Twenty SPF-SD female rats (5-6 weeks old) were equal y randomized into the control group and experimental group. In control group, the freshly isolated ovaries were fixed in formalin. In experimental group, the freshly isolated ovaries were vitrified immediately and cut into thin slices. After stored in liquid nitrogen for 21 days, the tissues of experimental group were rapidly thawed and transplanted into back muscles of rats for 2 or 4 weeks, respectively. After that, al rats in experimental group were sacrificed and the ovarian tissues were col ected and fixed in 4%formaldehyde solution. Then the ovarian tissues were stained with HE and observed under the light confocal microscope. Re-sults:With the naked eyes, there was no specific alteration except the size reduction with color changing. Under microscopy, we found normal cortex and medul a in the ovary, and the primordial fol icles and fol icles in various stages were observed in the cortex. The normal oocytes in ovarian tissues of experimental group were significant decreased than in the control group. Conclusion:The ovarian tissues survive wel in experimental group and there is no significant dif erence in the proportion of fol icles between dif erent times (2 and 4 weeks) after grafting. Our results suggest that thawed ovarian tissues could survive after heterotopic transplantation into back muscles of rat models and maintain their morphology and function.
作者： 刊期： 2014年第03期
《The Chinese-German Journal of Clinical Oncology (中德临床肿瘤学杂志)》是教育部主管、华中科技大学同济医学院主办的全英文国际性学术刊物。主要刊登肿瘤学领域的优秀科研成果和临床诊疗经验及基础理论研究方面的论文。
作者： 刊期： 2014年第03期
Imaging is now a wel established component of radiation therapy with al major imaging modalities represented and numerous examples in which these modalities have been used in treatment planning to al ow increased accuracy and precision in the delivery of dose. While these developments are wel established since a fairly long time for external beam radiotherapy, they are yet to be firmly established in many brachytherapy units al over the world particularly in the developing countries. We wil review the basic principles of image-based brachytherapy and explore the potential clinical opportunities behind this innovative technique.
作者： 刊期： 2014年第03期
作者： 刊期： 2014年第03期
Objective:The purpose of the study is to investigate the technical points, ef ects and complications of fiducial marker implantation within target areas before the CyberKnife treatment on body malignant solid tumors. Methods:Five hundred and four cases of patients with body malignant solid tumors accepted fiducial implantation within target areas under CT guidance before the treatment of CyberKnife. Observe the complications and ef ect. Results:Among the 504 cases, 500 cases successful y accepted the implantation (a success rate of 99.2%). 158 patients felt pain at the punctured sites and 3 patients had tachycardia. 33 patients had abdominal pain after the surgery due to a smal amount of bleeding in the needle passage during liver puncturing process. Among the 19 lung cancer patients who accepted lung paracentesis, 1 case had light pneumothorax and 1 case got light haemothorax. Among the 453 patients who accepted liver paracentesis, 6 had fiducial migration. Conclusion:The method of fiducial implantation within target areas before treating body malignant solid tumor with CyberKnife is minimal y invasive and comparatively secure.
作者： 刊期： 2014年第03期
Particle radiation therapy is an exciting area of radiotherapy basic and clinical researches. The majority of particle radiotherapy work is being done with proton beams having essential y the same radiobiologic properties as conventional photon/electron radiation but al owing a much more precise control of the radiation dose distribution. However, other charged particles are also playing an increasing role, like neutrons. In this review article we wil summarize the data related to basic and clinical experiences related to particle beam radiation therapy.
作者： 刊期： 2014年第03期
作者：《中德临床肿瘤学杂志》编辑部 刊期： 2014年第03期
Cystic liver metastasis, which lacks specific clinical manifestations and standardized treatment, rarely occurs after radical gastrectomy. This study presented two gastric cancer cases with cystic liver metastasis treated in Wuxi People’s Hospital from September 2008 to November 2012. The two female patients were 66 and 65 years old, respectively. Poorly dif erentiated ulcerated adenocarcinoma classified as stage III B [Tumor, Node, and Metastasis Classification (T4aN2M0)] and clinical manifestations of abdominal distension and ascites were detected in both patients. Cyst fluids were subjected to cy-tological analysis. Results confirmed the presence of liver metastasis of gastric cancer. The two patients benefited from local intracavitary chemotherapy or systemic intravenous chemotherapy.
作者： 刊期： 2014年第03期
作者： 刊期： 2014年第03期
Intraoperative radiotherapy (IORT) in its broadest sense refers to the delivery of radiation at the time of an opera-tion. It includes multiple techniques, namely intraoperative electron irradiation, intraoperative brachytherapy and intraopera-tive photon irradiation. It has a wide range of existing and potential y enlarging clinical applications. We wil discuss in this review the rationale for and use of intraoperative irradiation in conjunction with surgical exploration with or without external-beam irradiation (EBRT) and chemotherapy.
作者： 刊期： 2014年第03期
肿瘤学与转化医学（英文）杂志
主管:中华人民共和国教育部
主办：华中科技大学同济医院内
