学术投稿

大鼠大脑中动脉阻塞后DNA单链断裂损伤的实验研究

万琪;张巍;刘勇红

关键词:脑缺血, 模型, 动物, DNA, 单链, DNA聚合酶Ⅰ
摘要:目的观察大鼠永久性大脑中动脉阻塞( pMCAO)后,相应区域 DNA断裂损伤情况,探讨 DNA单链损伤在 pMCAO所致脑组织损伤中的意义.方法采用常规 HE组织染色明确大鼠 pMCAO模型成功与否及其损伤范围,采用 DNA聚合酶- 1 Klenow片段原位杂交方法检测 pMCAO不同时间点 DNA单链断裂损伤情况.结果损伤区域单链 DNA断裂的 Klenow阳性细胞在 pMCAO后 6 h即有表达( P< 0.01),在 24 h时达到高峰( P< 0.01), 72 h时有所下降( P< 0.01).结论单链 DNA断裂损伤在 pMCAO后 6 h即可出现,并逐渐积累,成为缺血半暗带内细胞损伤发展过程的重要环节,并可能预示半暗带损伤范围的不断扩大.
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中国组织工程研究杂志

中国组织工程研究杂志

主管:中华人民共和国卫生部

主办:中国康复医学会,《中国组织工程研究与临床康复》杂志社