Objective:To study the vitamin D3 levels in primary Sjogren's syndrome patients and its correlation with severity and immune function.Methods:Active pSS patients, stable pSS patients and healthy volunteers were chosen for study. Peripheral blood was collected, mononuclear cells were isolated and percentages of CD27high plasma cells and CD27+ memory B cells were detected; serum was collected and contents of 1,25(OH)2D3, TNF-α, IL-6, IL-10 and IL-17 were detected.Results:(1) 1,25(OH)2D3 contents in serum as well as CD27high plasma cell and CD27+ memory B cell contents in peripheral blood of pSS group were lower than those of control group; serum TNF-α, IL-6 and IL-17 contents were higher than those of control group and IL-10 contents were lower than those of control group; (2) 1,25(OH)2D3 contents in serum as well as CD27high plasma cell and CD27+ memory B cell contents in peripheral blood of active pSS patients were lower than those of stable pSS patients; serum TNF-α, IL-6 and IL-17 contents were higher than those of stable pSS patients and IL-10 contents were lower than those of stable pSS patients; (3) 1,25(OH)2D3 level was positively correlated with contents of CD27highplasma cells, CD27+ memory B cells and IL-10, and negatively correlated with TNF-α, IL-6 and IL-17 contents.Conclusion:Abnormal reduction of Vitamin D3 levels is involved in the pathogenesis of primary Sjogren's syndrome and closely related to the severity and immune function of the disease.

作者： 刊期： 2015年第11期

Objective:To study the hemodynamic condition of uterine artery and renal artery in preeclampsia patients and its relationship with endothelial function and invasive function. Methods:Preeclampsia puerperas were enrolled in observation group of the research, including 20 cases each with mild preeclampsia, moderate preeclampsia and severe preeclampsia; healthy puerperas were enrolled in control group. Then color Doppler ultrasound was used to detect hemodynamic parameters of uterine spiral artery and bilateral renal interlobar artery, enzyme-linked immunosorbent assay was used to detect endothelial function indexes in serum, and PCR was used to detect invasive function parameters in placenta.Results: S/D, PI and RI of uterine spiral artery and bilateral renal interlobar artery in mild, moderate and severe preeclampsia patients were all higher than those of control group; the more severe the preeclampsia condition was, the higher the S/D, PI and RI of uterine spiral artery and bilateral renal interlobar artery were; mRNA contents of Cst L, Cst D and MMP-9 in placenta of mild, moderate and severe preeclampsia patients were lower than those of control group, and mRNA contents of RECK as well as serum sFlt-1, sEng, AT1-AA and AngII contents were higher than those of control group; the more severe the disease degree was, the lower the mRNA contents of Cst L, Cst D, and MMP-9 were, the higher the mRNA contents of RECK as well as serum sFlt-1, sEng, AT1-AA and AngII contents were.Conclusion:Resistance of uterine artery and renal artery in preeclampsia patients increases, and it is closely related to endothelial dysfunction and invasive function loss.

作者： 刊期： 2015年第11期

Objective:To observe and analyze the change situation of immune indexes and oxidative stress state of children with recurrent respiratory tract infection.Methods:75 children with recurrent respiratory tract infection in our hospital from January 2014 to June 2015 were selected as observation group, 75 healthy children with health examination at the same time were selected as control group, then the immune indexes and oxidative stress state related serum indexes of two groups were detected,and the detection results of observation group with boys and girls, mild, moderate and severe disease were compared.Results: The cellular immune and erythrocyte immune and oxidative stress state related serum indexes of observation group were all worse than those of control group,and the detection results of observation group with mild, moderate and severe disease had obvious differences too, allP<0.05, while the differences of boys and girls had no significant differences, allP>0.05.Conclusion:The change of immune indexes and oxidative stress state of children with recurrent respiratory tract infection are obvious,and the differences of children with mild, moderate and severe disease are obvious too,while the differences of boys and girls are not obvious.

作者： 刊期： 2015年第11期

Objective:To explore the effect and mechanism of ganglioside for hypoxia injury in newborn.Methods:A total of 200 cases of newborn patients in our hospital were analyzed. All the patients were divided into 4 groups, control and ganglioside groups with low, medium and high dose. The neonatal behavioral neurological assessment scores were counted in each group and the serum inflammatory factors and transforming growth factor-β (TGFβ)/Smad parameters were detected.Results:The neonatal behavioral neurological assessment scores were increased after ganglioside treatment and the serum interleukin (IL) 2/10, hypoxia inducible factorαand Smad1, Smad3 as well as TGFβ was decreased dramatically when compared with control group.Conclusions:Ganglioside exerts an effective effect on cerebral hypoxia in newborn mainly by inhibiting the TGFβ/Smad signaling pathway.

作者： 刊期： 2015年第11期

Objective:To explore the value of arterial blood gas analysis and electrolyte determination in the diagnosis of neonatal asphyxia.Methods: A total of 100 neonates with asphyxia who were admitted in our department from March, 2013 to March, 2014 were included in the study and divided into the mild asphyxia group and the severe asphyxia group according to Apgar scoring. Moreover, 50 normal neonates were served as the control group and used for comparative analysis. AVL blood-gas analyzer was used to detect the levels of pH, PaO2, HCO3-, BE, PaCO2, K+, Na+, Cl-, and Ca2+.Results:The concentrations of pH, PaO2, HCO3-, and BE in the severe asphyxia group were significantly lower than those in the mild asphyxia group and the control group, while PaCO2 level was significantly higher than that in the mild asphyxia group and the control group. The comparison of the various indicators between the control group and the mild asphyxia group was not statistically significant. The comparison of serum K+, Na+,and Cl- levels among the control group, the mild asphyxia group, and the severe asphyxia group was not statistically significant. The serum Ca2+ level in the severe asphyxia group was significantly lower than that in the mild asphyxia group and the control group, while the comparison between the mild asphyxia group and the control group was also statistically significant.Conclusions:Blood gas analysis and electrolyte determination to the arterial blood in neonates can compensate for the insufficiency of Apgar scoring and provide an objective evidence for the diagnosis of neonatal asphyxia and the estimation of severity degree. Clinical combination with Apgar scoring is of great significance in enhancing the diagnosis of neonatal asphyxia and the accuracy of severity evaluation.

作者： 刊期： 2015年第11期

Objective:To observe and study the influence degree of hysteroscope examination for the comprehensive stress state of patients with recurrent spontaneous abortion.Methods:Seventy patients with recurrent spontaneous abortion who were intervened with hysteroscope examination in our hospital from January 2013 to April 2015 were selected as the study object, and they were divided into Group A (normal uterine cavity group, 35 cases) and Group B (intrauterine lesions group, 35 cases) according to the final examination results. Then the blood expression levels of stress hormones and pain stress indexes of two groups before the examination, at 5 min during the examination and at 5 min after the examination were detected, and the detection results of two groups at different times were compared.Results:The blood expression levels of stress hormones and pain stress indexes of Group B before the examination, at 5 min during the examination and at 5 min after the examination were all higher than those of Group A. And the blood expression levels of two groups at 5 min during the examination were all higher than those before the examination and at 5 min after the examination. The differences were all significant.Conclusions:The influence of hysteroscope examination for the comprehensive stress state of patients with recurrent spontaneous abortion is obvious, and it should be paid more attention to the control of stress degree of patients with intrauterine lesions during operation.

作者： 刊期： 2015年第11期

Objective:To explore the correlation of expressions of MDR1/P-gp and CerbB-2 in the colorectal cancer tissues and their clinical significance.Methods:A total of 86 colorectal cancer tissues specimens were included in the study. The immunohistochemical S-P method was used to detect the expressions of P-gp and CerbB-2, and their correlations were analyzed.Results:The positive rates of the expressions of MDR1/P-gp and CerbB-2 were 36% (31/86) and 28% (24/86), respectively. The positive expression rate of CerbB-2 in the colorectal cancer tissues at a clinical stage of III was significantly higher than that at stage I and II. The positive expression rates of MDR1/P-gp and CerbB-2 in the colorectal cancer tissues with axillary lymph node metastasis were significantly higher than those in the tissues without axillary lymph node metastasis. In the P-gp positive expression group, the positive rate of CerbB-2 was 75.5% (37/49), while the negative rate of CerbB-2 was 24.48% (12/49), and the difference was statistically significant (P<0.01). P-gp had a certain positive correlation with the positive expression rate of CerbB-2. Conclusions:P-gp and CerbB-2 play a certain role in the occurrence of multidrug resistance of colorectal cancer, and their expressions are correlated; therefore, the combination detection can provide an evidence for the chemotherapy choice of colorectal cancer.

作者： 刊期： 2015年第11期

Objective:To explore the feasibility and effectiveness of 64 slice spiral CT in evaluating the patency of coronary artery after stent implantation.Methods:The 64 slice spiral CT image data of 125 patients after coronary artery stent implantation were collected, meanwhile, the image data of 25 patients underwent coronary angiography were also collected. The feasibility and accuracy of 64 slice spiral CT coronary artery stent imaging were comparatively analyzed. Results: The 64 slice spiral CT imaging quality with a stent diameter greater than 3.00 mm was significantly superior to that with a diameter of 2.25-3.00 mm. The CT imaging quality in the left main coronary artery and anterior descending artery was significantly higher than that in the left circumflex coronary artery. The CT imaging quality in the left main coronary artery was significantly higher than that in the right coronary artery. The CT reconstruction imaging quality in the drug coating stent was significantly superior to that in the bare metal stent. The sensitivity of 64 slice spiral CT was 100.00%, and the accuracy was 100.00%. In detecting the coronary artery with occlusion and stenosis (stent stenosis greater than 50%), the sensitivity was 90.00%, the false negative rate was 10.00%, and the positive predicative value was 100.00%.Conclusions:The effect of 64 slice spiral CT coronary imaging in evaluating the patency of coronary artery after stent implantation is highly consistent with that by coronary angiography, with a simple operation, less risk, and low cost, and thus, it can be completely taken as the imaging method in evaluating the patency of coronary artery after stent implantation.

作者： 刊期： 2015年第11期

Objective:To explore the effect of caffeine citrate on the blood gas indexes in newborns with apnea.Methods: A total of 108 primary premature infants diagnosed with apnea were included in the study and randomized into the caffeine citrate treatment group and the aminophylline control group with 54 cases in each group. The apnea episode duration 72 h after medication, and the blood gas before medication and 24, 48, 72 h after medication between the two groups were compared.Results: The apnea episode duration 24, 48, 72 h after medication in the treatment group was significantly shorter than that in the control group. PaO2 24, 48, 72 h after medication in the treatment group was significantly higher than that in the control group, while PaCO2 was significantly reduced when compared with the control group.Conclusions:Caffeine citrate has achieved a significantly clinical effect in the treatment of newborn apnea, as a new therapeutic drug, is worthy of being widely recommended in the clinic.

作者： 刊期： 2015年第11期

Objective:To explore the impact of serum free amino acid and cytokines in patients with tuberculosis treated by Moxifloxacin.Methods:Chose 130 cases pulmonary tuberculosis patients,they were divided into observation group and control group according to random number table method, 65 cases in each group, all patients were given tuberculosis standard treatment, and on this basis, patients in control group were given levofloxacin tablets, patients in observation group were given moxifloxacin hydrochloride oral, they were treated for 6 months, compared the serum free amino acid and cytokine interleukin-18 (IL-18), tumor necrosis factor alpha-gamma (TNF-α), interferon (IFN-γ), interleukin-10 (IL-10) between two groups before and after treatment. The serum isoleucine, phenylalanine, threonine, leucine, valine, aspartic acid, glutamic acid and arginine in two groups after were increased significantly than before treatment, the difference was statistically significant, acquired aspartic acid, glutamic acid in observation group after treatment were significantly higher than the control group after treatment, the difference was statistically significant; The serum IL-18, IFN-γ, TNF-α and IL-10 in two groups after treatment were significantly reduced, the difference was statistically significant (P<0.05); the serum IL-18, IFN-γ, TNF-α and IL-10 in observation group after treatment were significantly lower than the control group after treatment, the difference was statistically significant.Conclusion:Compared with levofloxacin, moxifloxacin treatment tuberculosis can improve the patients' serum free amino acid levels, adjust the Th1/Th2 balance.

作者： 刊期： 2015年第11期

Objective:To explore the expression change of serum and colon lavage fluid related indicators after probiotics combined with zinc preparations treatment of persistent diarrhea in children.Methods:80 cases of children with diarrhea treated in our hospital were selected. According to clinical symptoms, routine blood and stool test, they were all diagnosed with persistent diarrhea and randomly divided into control group and experimental group, 40 cases in each group. Control group received basic treatment and zinc preparations (zinc gluconate tablets) therapy; experimental group, based on treatment of control group, received probiotics (Siliankang) combined with zinc preparations treatment. Fasting serum and colon lavage fluid of both groups were collected before and after treatment to detect IL-6, IL-8, NO, MDA and SOD expression levels, and differences between two groups were compared.Results:After treatment, IL-6 and IL-8 expression levels in serum and colon lavage fluid of both groups decreased significantly than those before treatment, and those of experimental group decreased more significantly; after treatment, NO, MDA and SOD expression levels in serum and colon lavage fluid of both groups were significantly improved than those before treatment, and those of experimental group were closer to the normal levels than those of control group.Conclusions:Probiotics combined with zinc preparations treatment of persistent diarrhea in children has obvious curative effect; it effectively reduces expression levels of inflammatory cytokines and oxidative stress factors in serum and colon lavage fluid, and is worth popularization in future clinical treatment.

作者： 刊期： 2015年第11期

Objective:To evaluate the influence of neoadjuvant chemotherapy for the microRNA and tumor-related indicators of patients with advanced breast cancer.Methods: 120 cases of patients with advanced breast cancer were randomly divided into two groups, NC group and CC group, each group had 60 cases, and 60 cases of patients with benign breast disease and healthy volunteers in the same period were included as BC group and HC group. Then the plasma levels of miR-31, miR-200c, miR-205 and sIL-2R, IL-6, VEGF of all subjects were detected and compared.Results:The plasma levels of miR-31 and miR-205 of NC group and CC group before treatment were lower than BC group and HC group, while the miR-200c and sIL-2R, IL-6, VEGF were higher than BC group and HC group. The efficacy of treatment of advanced breast cancer patients was positively correlated to the plasma levels of miR-31, miR-205 expression, and negatively correlated to the plasma levels of miR-200c and sIL-2R, IL-6, VEGF. After 15, 45 days of chemotherapy, the plasma levels of miR-31, miR-205 expression of NC group were significantly higher than CC group, and miR-200c and sIL-2R, IL-6, VEGF were significantly lower than CC group. Conclusion:Neoadjuvant chemotherapy can effectively increase the plasma levels of miR-31, miR-205, and down the plasma levels of miR-200c and sIL-2R, IL-6, VEGF, will beneficial to improve the advanced breast cancer.

作者： 刊期： 2015年第11期

Objective:To study the miRNA-21 content in cervical cancer tissue and analyze its downstream target molecules.Methods:Patients with different FIGO stages of cervical cancer and healthy subjects were selected, cervical cancer tissue and normal cervical tissue were collected, and contents of miRNA-21 and apoptotic genes were detected; cervical cancer SiHa cells were cultured, miRNA-21 mimics and inhibitors were transfected, and then apoptotic gene contents were detected.Results:miRNA-21 contents in different stages of cervical cancer tissue were all higher than those in normal cervical tissue, mRNA contents of p16ink4a, ASPP1, Fas and GRIM-19 were lower than those in normal tissue, and mRNA contents of p16ink4a, ASPP1, Fas and GRIM-19 were negatively correlated with miRNA-21 contents; after miRNA-21 mimics were transfected, mRNA contents of p16ink4a, ASPP1, Fas and GRIM-19 significantly decreased, and after miRNA-21 inhibitors were transfected, mRNA contents of p16ink4a, ASPP1, Fas and GRIM-19 significantly increased.Conclusion:miRNA-21 contents in cervical cancer tissue significantly increase; downstream target genes of this miRNA may be apoptotic genes p16ink4a, ASPP1, Fas and GRIM-19.

作者： 刊期： 2015年第11期

Objective:To study the protective effect of diammonium glycyrrhizinate enteric-coated capsule on hepatic injury of leukemia tumor-bearing mice after chemotherapy.Methods: C57 mice were selected as research subjects, leukemia tumor-bearing mouse model was built, both chemotherapy group and treatment group received cyclophosphamide chemotherapy, and treatment group received diammonium glycyrrhizinate enteric-coated capsule treatment. Then liver enzyme contents in serum as well as percentage of apoptosis cells and oxidative stress product contents in liver tissue were detected.Results:Serum ALT, AST,α-GST and GLDH contents of chemotherapy group were higher than those of control group, and percentage of apoptosis cells as well as MDA, AOPP and 8-OhdG contents in liver tissue were higher than those of control group; serum ALT, AST,α-GST and GLDH contents of treatment group were lower than those of chemotherapy group, and percentage of apoptosis cells as well as MDA, AOPP and 8-OhdG contents in liver tissue were lower than those of chemotherapy group.Conclusion:diammonium glycyrrhizinate enteric-coated capsule has protective effect on hepatic injury of leukemia tumor-bearing mice after chemotherapy; mechanism of the protective effect may be reducing the oxidative stress and inhibiting hepatocyte apoptosis.

作者： 刊期： 2015年第11期

Objective: To explore the effect of different anesthesia and analgesia methods on the T lymphocyte subsets, cytokines and the levels of the stress hormone in patients with breast cancer surgery.Methods: 86 cases of breast cancer were divided into two groups by random digits table, control group of 43 cases with total intravenous anesthesia, and the observation group of 43 cases with total intravenous anesthesia combined with epidural anesthesia and postoperative underwent analgesia. The T lymphocyte subsets, cytokines and the levels of the stress hormone were detected before and after operation and compared. Results:CD3+, CD4+ and CD4+/CD8+ of the two groups at T1 or T2 were significantly lower than those at T0, and those in the observation group were significantly lower than the control group, and the difference between the two groups has statistical significance. IL-6, CRP and TNF-α levels of the two groups at T1, T2 or T3 were significantly higher than those at T0, and those in the observation group were significantly lower than the control group, and the difference between the two groups has statistical significance. Each index of two groups at T4 were restored to T0, and the differences were no statistically significant. The cortisol levels of two groups at T1 or T2 were significantly increased compared with T0, and the increase in the observation group was less than that of the control group, the difference between the two groups has statistical significance. The cortisol levels of two groups at T4 were restored to T0, and the differences were no statistically significant.Conclusion:Epidural analgesia after the intravenous anesthesia combined with epidural anesthesia for breast cancer cure patients has lighter immunosuppression and stress reaction, has less influence on inflammatory factors, is an ideal anesthesia and analgesia.

作者： 刊期： 2015年第11期

Objective:To explore the clinical effect and significance of adoptive immunotherapy of dendritic cell and cytokine-induced killer cell (DC-CIK) combined with the gamma knife in the treatment of middle and advanced hepatic carcinoma.Methods:42 patients with the middle and advanced primary hepatic carcinoma were randomly divided into two groups: 20 cases in the combination group were given the adoptive immunotherapy of DC-CIK cells and gamma knife radiotherapy; 22 cases in the control group were only given the gamma knife radiotherapy. The short-term effect, quality of life, overall survival and toxic and side effects were compared between two groups after the operation.Results: 3 months after the treatment, the short-term effect of combination group and control group was 70% and 54.5% respectively (P<0.05). Patients in the combination group performed better in the overall survival, change of T-cell subsets, PS score, decrease rate of AFP and degree of liver function than the control group, while the adiodermatitis at II and over and bone marrow suppression were also better than the control group. Conclusion:The adoptive immunotherapy of DC-CIK cells combined with the gamma knife in the treatment of middle and advanced hepatic carcinoma can prolong the overall survival, improve the quality of life, reduce the toxic and side effect and effectively promote the short-term clinical effect for patients.

作者： 刊期： 2015年第11期

Objective:To explore the effect of acitretin in combined with NB-UVB on T lymphocyte subsets in the peripheral blood in patients with psoriasis vulgaris.Methods:A total of 90 patients with psoriasis vulgaris who were admitted in our department from May, 2013 to May, 2015 were included in the study and randomized into the observation group and the control group. The patients in the control group were given acitretin after dinner, 10 mg/time, twice each day, for 6 weeks. The patients in the observation group were given acitretin in combined with UVB, each irradiation for 30 min, three times every week, for 6 weeks. The changes of CD3+, CD4+, and CD8+ in the peripheral blood, PASI score, and the clinical efficacy before and after treatment in the two groups were observed.Results:The elevation degree of CD3+ and CD4+ after treatment in the observation group was significantly obvious that in the control group, while the declining degree of CD8+ was also significantly obvious that in the control group. The decreased degree of PASI score after treatment in the observation group was significantly superior to that in the control group. The total effective rate in the observation group (93.33%) was significantly superior to that in the control group (80.00%).Conclusions:Acitretin in combined with UVB in the treatment of psoriasis vulgaris can play its role by altering the imbalance state of T lymphocyte immune function. The combined treatment can enhance the therapeutic effect, therefore, it deserves to be widely recommended in the clinic.

作者： 刊期： 2015年第11期

Objective:To discuss the clinical value of Serum TSH combined with 3 kinds of VEGF (VEGF-C, VEGF-D and VEGFR-3) determination in the early diagnosis of papillary thyroid carcinoma (PTC). Method:Selected 37 cases of patients with thyroid benign tumor (Benign group) and 37 cases of patients with PTC (PTC group), then collected the serum of these both groups, to determine the TSH, VEGF-C, VEGF-D and VEGFR-3 levels of all cases by chemiluminescence immunoassay and enzyme linked immunosorbent assay respectively. Through Logistic model, to calculate the curve area of TSH combined with 3 kinds of VEGF.Results:PTC group: VEGF-C, VEGFR-3 and TSH levels were obviously higher than that in Benign group (P<0.05); and VEGF-C, VEGFR-3 and TSH levels inⅢ-Ⅳ period patients were obviously higher than that in I-Ⅱ period patients (P<0.05); AUC area of VEGF-C, VEGFR-3 and TSH were respectively 0.805, 0.736 and 0.710, reached to significance level (P<0.05); AUC area of combined diagnosis was 0.859.Conclusion:VEGF-C, VEGFR-3 and TSH between papillary thyroid carcinoma and thyroid benign tumor had significant difference. Combined determination could improve the early diagnose rate of PTC, and could be regarded as one of the important auxiliary index of PTC early diagnosis.

作者： 刊期： 2015年第11期

Objective:To explore the diagnostic value of the combined detection ofβ2-MG, AFP, SF, and CEA in the primary hepatic cancer (PHC).Methods:A total of 70 HPC patients, 30 hepatic cirrhosis patients, and 30 healthy individuals were included in the study. A volume of 3 mL morning fasting blood from the patients in the three groups was taken. The radioimmunoassay was used to detect the contents of serumβ2-MG, AFP, SF, and CEA.Results: The contents of serumβ2-MG, AFP, SF, and CEA in the PHC group were significantly higher than those in the hepatic cirrhosis group and the control group (P<0.05). The contents of serumβ2-MG, AFP, and CEA in the hepatic cirrhosis group were significantly higher than those in the control group (P<0.05). The comparison of serum SF content between the hepatic cirrhosis group and the control group was not statistically significant. The detection positive rates of serumβ2-MG, AFP, SF, and CEA in the PHC patients were 81.42%, 65.71%, 51.43%, and 12.86%, respectively. The positive rate by the combined detection of four tumor markers could reach 97.14%, and was significantly higher than that by a unique detection. The comparison of serumβ2-MG and AFP in the PHC patients was not comparable.Conclusions:The contents of serumβ2-MG, AFP, SF, and CEA in the PHC patients are significantly higher than those in the hepatic cirrhosis patients and the healthy individuals. The combined detection of serumβ2-MG, AFP, SF, and CEA can contribute for the screening of PHC, and reduce the missed diagnosis rate of PHC. Serumβ2-MG is an independent tumor marker with no relation to AFP, SF, and CEA.

作者： 刊期： 2015年第11期

Objective:To explore the influence of inflammation factors and intestinal mucosal barrier function on severe acute pancreatitis patients treated with bifidobacterium triple viable capsules.Methods:88 cases severe acute pancreatitis patients were divided into observation group and control group according to admission order, 44 cases in each group, all patients were given conventional treatment, on this base, patients in observation group were treated by oral bifidobacterium triple viable capsules, they were treated for one week, detected the serum inflammatory factors: the tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6) and C-reactive protein (CRP) and intestinal mucosal barrier function index: endotoxin, diamine oxidase (DAO), D-lactic acid, urinary lactulose/mannitol (L/M) in the two groups before and after treatment.Results:The levels of TNF-α, IL-6 and CRP in two groups after treatment were significantly reduced than before treatment, and after treatment the levels of TNF-α, IL-6 and CRP in observation group was significantly lower than that of the control group, all the difference was statistically significant; The levels of endotoxin, DA, D-lactic acid, L/M in two groups after treatment were significantly reduced than before treatment, and the levels of endotoxin, DA, D-lactic acid,L/M after treatment in observation group was significantly lower than that of the control group, the difference was statistically significant.Conclusion: Based on conventional treatment combined bifidobacterium triple viable capsules can significantly reduce inflammation in patients with severe acute pancreatitis, it can protect the intestinal mucosal barrier function, and has important clinical significance.

作者： 刊期： 2015年第11期