Objective:To investigate and study the influenced state of total laparoscopic hysterectomy for the blood viscosity and erythrocyte immune state of patients.Methods:A total of 58 patients who were treated with total hysterectomy in our hospital from June 2013 to February 2015 were the study subjects, the 29 cases in the control group were treated with routine open hysterectomy operation according to the operation types, the 29 cases in the observation group were treated with laparoscopic hysterectomy operation, then the preoperative and postoperative blood viscosity and erythrocyte immune state indexes of two groups were compared.Results:The blood viscosity indexes of the observation group at different postoperative time were all lower than the indexes of the control group, the erythrocyte immune state indexes were all better than those of the control group, and the detection results of two groups at the third day after the operation were all obviously worse than those at other times, their differences were statistically significant.Conclusions:The bad influence of total laparoscopic hysterectomy for the blood viscosity and erythrocyte immune state of patients are relatively smaller, and those postoperative indexes in recovery of patients are faster.

作者： 刊期： 2015年第11期

Objective:To observe the effect of atorvastatin combined with ezetimibe on the levels of urine protein, PCT, serum lipid, and hs-CRP in patients with acute coronary syndrome (ACS). Methods:A total of 90 ACS patients who were admitted in our hospital were included in the study and randomized into A, B, and C groups with 30 cases in each group. The patients in A group were given atorvastatin (20 mg/d, a maintenance dose), the patients in B group were given atorvastatin (40 mg/d, a load dose), while the patients in C group were given atorvastatin in a load dose combined with ezetimibe (10 mg/d). The changes of 24 h UMA, PCT, serum lipid, and hs-CRP after two-week treatment in the three groups were observed, and the adverse reactions in the three groups were observed.Results:The improvements of urine protein, PCT, and serum hs-CRP concentration after treatment in B and C groups were significantly superior to those in A group (P<0.05). The improvements of urine protein, PCT, and serum hs-CRP concentration after treatment in C group were significantly superior to those in A and B groups (P<0.05). The levels of TC and LDL-C after treatment in B and C groups were significantly reduced when compared with those before treatment (P<0.05). The levels of TC and LDL-C after treatment in group A were not statistically different from those before treatment (P>0.05). No obviously adverse reactions occurred in the three groups.Conclusions:Atorvastatin in a load dose combined with ezetimibe can significantly adjust the levels of UMA, PCT, and serum lipid in ACS patients, reduce the concentrations of inflammatory factors, and play an active role in the treatment of ACS.

作者： 刊期： 2015年第11期

Objective:To explore the effect of acitretin in combined with NB-UVB on T lymphocyte subsets in the peripheral blood in patients with psoriasis vulgaris.Methods:A total of 90 patients with psoriasis vulgaris who were admitted in our department from May, 2013 to May, 2015 were included in the study and randomized into the observation group and the control group. The patients in the control group were given acitretin after dinner, 10 mg/time, twice each day, for 6 weeks. The patients in the observation group were given acitretin in combined with UVB, each irradiation for 30 min, three times every week, for 6 weeks. The changes of CD3+, CD4+, and CD8+ in the peripheral blood, PASI score, and the clinical efficacy before and after treatment in the two groups were observed.Results:The elevation degree of CD3+ and CD4+ after treatment in the observation group was significantly obvious that in the control group, while the declining degree of CD8+ was also significantly obvious that in the control group. The decreased degree of PASI score after treatment in the observation group was significantly superior to that in the control group. The total effective rate in the observation group (93.33%) was significantly superior to that in the control group (80.00%).Conclusions:Acitretin in combined with UVB in the treatment of psoriasis vulgaris can play its role by altering the imbalance state of T lymphocyte immune function. The combined treatment can enhance the therapeutic effect, therefore, it deserves to be widely recommended in the clinic.

作者： 刊期： 2015年第11期

Objective:To explore the effect of caffeine citrate on the blood gas indexes in newborns with apnea.Methods: A total of 108 primary premature infants diagnosed with apnea were included in the study and randomized into the caffeine citrate treatment group and the aminophylline control group with 54 cases in each group. The apnea episode duration 72 h after medication, and the blood gas before medication and 24, 48, 72 h after medication between the two groups were compared.Results: The apnea episode duration 24, 48, 72 h after medication in the treatment group was significantly shorter than that in the control group. PaO2 24, 48, 72 h after medication in the treatment group was significantly higher than that in the control group, while PaCO2 was significantly reduced when compared with the control group.Conclusions:Caffeine citrate has achieved a significantly clinical effect in the treatment of newborn apnea, as a new therapeutic drug, is worthy of being widely recommended in the clinic.

作者： 刊期： 2015年第11期

Objective:To explore the effect of rosuvastatin on serum inflammatory factors, oxidative stress and MPO, MMP-9 of patients with chronic heart failure (CHF).Methods:140 cases with CHF were selected as the study case, using the single blind method and were randomly divided into two groups, including 70 cases in the control group were treated with routine department of internal medicine treatment, while 70 patients in the observation group were treated with rosuvastatin on the basis of conventional department. The serum inflammatory factors, oxidative stress and MPO, MMP-9 of changes were detected and compared.Results:After treatment, the observation group in patients with LVEDD (36.62±2.56) mm was significantly reduced compared with before treatment, LVEF (57.22±2.80)% and E/A ratio (1.38±0.04) were significantly increased compared with before treatment, and the each index compared with the control group had significant difference. After treatment, the observation group in patients with IL-6 (5.44±0.74) ng/L, TNF alpha (3.44±0.43) ng/L and hs-CRP (18.82±2.10) mg/mL compared with those before treatment significantly reduced, and the each index compared with the control group had significant difference (P<0.05). After treatment, the observation group in patients with MDA (4.78±0.60) μmol/mL, MPO (481.14±22.22) mg/L and MMP-9 (62.30±6.33) ng/mL was significantly reduced compared with before treatment, SOD (125.35±6.43) U/mL compared with increased significantly, and the each index compared with the control group had significant difference.Conclusion:Conventional therapy combined with rosuvastatin on the effect of CHF is significant, and can significantly improve heart function, inhibit oxidative stress and anti-inflammation, and the clinical application value is higher.

作者： 刊期： 2015年第11期

Objective:To explore the expression change of serum and colon lavage fluid related indicators after probiotics combined with zinc preparations treatment of persistent diarrhea in children.Methods:80 cases of children with diarrhea treated in our hospital were selected. According to clinical symptoms, routine blood and stool test, they were all diagnosed with persistent diarrhea and randomly divided into control group and experimental group, 40 cases in each group. Control group received basic treatment and zinc preparations (zinc gluconate tablets) therapy; experimental group, based on treatment of control group, received probiotics (Siliankang) combined with zinc preparations treatment. Fasting serum and colon lavage fluid of both groups were collected before and after treatment to detect IL-6, IL-8, NO, MDA and SOD expression levels, and differences between two groups were compared.Results:After treatment, IL-6 and IL-8 expression levels in serum and colon lavage fluid of both groups decreased significantly than those before treatment, and those of experimental group decreased more significantly; after treatment, NO, MDA and SOD expression levels in serum and colon lavage fluid of both groups were significantly improved than those before treatment, and those of experimental group were closer to the normal levels than those of control group.Conclusions:Probiotics combined with zinc preparations treatment of persistent diarrhea in children has obvious curative effect; it effectively reduces expression levels of inflammatory cytokines and oxidative stress factors in serum and colon lavage fluid, and is worth popularization in future clinical treatment.

作者： 刊期： 2015年第11期

Objective:To observe and study the influence degree of hysteroscope examination for the comprehensive stress state of patients with recurrent spontaneous abortion.Methods:Seventy patients with recurrent spontaneous abortion who were intervened with hysteroscope examination in our hospital from January 2013 to April 2015 were selected as the study object, and they were divided into Group A (normal uterine cavity group, 35 cases) and Group B (intrauterine lesions group, 35 cases) according to the final examination results. Then the blood expression levels of stress hormones and pain stress indexes of two groups before the examination, at 5 min during the examination and at 5 min after the examination were detected, and the detection results of two groups at different times were compared.Results:The blood expression levels of stress hormones and pain stress indexes of Group B before the examination, at 5 min during the examination and at 5 min after the examination were all higher than those of Group A. And the blood expression levels of two groups at 5 min during the examination were all higher than those before the examination and at 5 min after the examination. The differences were all significant.Conclusions:The influence of hysteroscope examination for the comprehensive stress state of patients with recurrent spontaneous abortion is obvious, and it should be paid more attention to the control of stress degree of patients with intrauterine lesions during operation.

作者： 刊期： 2015年第11期

Objective:To explore molecular mechanism and biological function of miR-155 and its target genes on endometriosis.Methods: The expression of miR-155 in Ems patient and healthy control were assayed by RT-PCR. After miR-155 mimic and inhibitor were transfected into Ems endometrial cells for 48 h, the viability of cell was detected by MTT assay. Transwell migration and invasion assay were used to detect cell migration and invasion. The expression of cell apoptotic protein Bax and Bcl-2, matrix metalloproteinase (MMP 2) and MMP 9 were assayed by western blot.Results: The expression of miR-155 in Ems patient was more than that in the health control (P<0.01). After miR-155 mimic and inhibitor were transfected into Ems endometrial cells for 48 h, miR-155 over-expression could increase cell viability, and promoted cell migration and invasion, which was related to down-regulation of Bax along with up-regulation of Bcl-2, MMP 2 and MMP 9.Conclusion:These results suggested miR-155 lower expression inhibit endometrial cell proliferation and migration of the Ems.

作者： 刊期： 2015年第11期

Objective:To explore the value of arterial blood gas analysis and electrolyte determination in the diagnosis of neonatal asphyxia.Methods: A total of 100 neonates with asphyxia who were admitted in our department from March, 2013 to March, 2014 were included in the study and divided into the mild asphyxia group and the severe asphyxia group according to Apgar scoring. Moreover, 50 normal neonates were served as the control group and used for comparative analysis. AVL blood-gas analyzer was used to detect the levels of pH, PaO2, HCO3-, BE, PaCO2, K+, Na+, Cl-, and Ca2+.Results:The concentrations of pH, PaO2, HCO3-, and BE in the severe asphyxia group were significantly lower than those in the mild asphyxia group and the control group, while PaCO2 level was significantly higher than that in the mild asphyxia group and the control group. The comparison of the various indicators between the control group and the mild asphyxia group was not statistically significant. The comparison of serum K+, Na+,and Cl- levels among the control group, the mild asphyxia group, and the severe asphyxia group was not statistically significant. The serum Ca2+ level in the severe asphyxia group was significantly lower than that in the mild asphyxia group and the control group, while the comparison between the mild asphyxia group and the control group was also statistically significant.Conclusions:Blood gas analysis and electrolyte determination to the arterial blood in neonates can compensate for the insufficiency of Apgar scoring and provide an objective evidence for the diagnosis of neonatal asphyxia and the estimation of severity degree. Clinical combination with Apgar scoring is of great significance in enhancing the diagnosis of neonatal asphyxia and the accuracy of severity evaluation.

作者： 刊期： 2015年第11期

Objective:To explore the feasibility and effectiveness of 64 slice spiral CT in evaluating the patency of coronary artery after stent implantation.Methods:The 64 slice spiral CT image data of 125 patients after coronary artery stent implantation were collected, meanwhile, the image data of 25 patients underwent coronary angiography were also collected. The feasibility and accuracy of 64 slice spiral CT coronary artery stent imaging were comparatively analyzed. Results: The 64 slice spiral CT imaging quality with a stent diameter greater than 3.00 mm was significantly superior to that with a diameter of 2.25-3.00 mm. The CT imaging quality in the left main coronary artery and anterior descending artery was significantly higher than that in the left circumflex coronary artery. The CT imaging quality in the left main coronary artery was significantly higher than that in the right coronary artery. The CT reconstruction imaging quality in the drug coating stent was significantly superior to that in the bare metal stent. The sensitivity of 64 slice spiral CT was 100.00%, and the accuracy was 100.00%. In detecting the coronary artery with occlusion and stenosis (stent stenosis greater than 50%), the sensitivity was 90.00%, the false negative rate was 10.00%, and the positive predicative value was 100.00%.Conclusions:The effect of 64 slice spiral CT coronary imaging in evaluating the patency of coronary artery after stent implantation is highly consistent with that by coronary angiography, with a simple operation, less risk, and low cost, and thus, it can be completely taken as the imaging method in evaluating the patency of coronary artery after stent implantation.

作者： 刊期： 2015年第11期

Objective:To study the hemodynamic condition of uterine artery and renal artery in preeclampsia patients and its relationship with endothelial function and invasive function. Methods:Preeclampsia puerperas were enrolled in observation group of the research, including 20 cases each with mild preeclampsia, moderate preeclampsia and severe preeclampsia; healthy puerperas were enrolled in control group. Then color Doppler ultrasound was used to detect hemodynamic parameters of uterine spiral artery and bilateral renal interlobar artery, enzyme-linked immunosorbent assay was used to detect endothelial function indexes in serum, and PCR was used to detect invasive function parameters in placenta.Results: S/D, PI and RI of uterine spiral artery and bilateral renal interlobar artery in mild, moderate and severe preeclampsia patients were all higher than those of control group; the more severe the preeclampsia condition was, the higher the S/D, PI and RI of uterine spiral artery and bilateral renal interlobar artery were; mRNA contents of Cst L, Cst D and MMP-9 in placenta of mild, moderate and severe preeclampsia patients were lower than those of control group, and mRNA contents of RECK as well as serum sFlt-1, sEng, AT1-AA and AngII contents were higher than those of control group; the more severe the disease degree was, the lower the mRNA contents of Cst L, Cst D, and MMP-9 were, the higher the mRNA contents of RECK as well as serum sFlt-1, sEng, AT1-AA and AngII contents were.Conclusion:Resistance of uterine artery and renal artery in preeclampsia patients increases, and it is closely related to endothelial dysfunction and invasive function loss.

作者： 刊期： 2015年第11期

Objective:To study the correlation of HIFs/PPAR signaling pathway activation degree and lipid metabolism in liver tissue of alcoholic fatty liver rat model.Methods:Adult SD rats were selected and alcoholic fatty liver rat models were established by alcohol administration and high-fat diet feeding. Liver tissue was collected and contents of HIF-1α, PPARγ and lipid metabolism-related enzymes were detected; serum was collected and contents of lipid metabolism indexes and liver cell damage indexes were detected.Results:(1) one week, two weeks, three weeks and four weeks after models were established, HIF-1αα in livers of the model group showed an increasing trend and PPARγ showed a decreasing trend; HIF-1α content was higher than that of the control group and PPARγ content was lower than that of the control group; (2) contents of apoCII, apoCIII,α-GST and GLDH in serum as well as levels of FAT, FABP1, FAS, ACC and ACAT-2 in liver tissue of the model group all significantly increased, and were positively correlated with HIF-1α and negatively correlated with PPARγ.Conclusion:Transcription factor HIF-1α content abnormally increases and PPARγ content abnormally decreases in liver tissue of alcoholic fatty liver rat models; it results in abnormal lipid metabolism and liver cell damage through increasing the expression of lipid metabolism-related enzymes in the liver.

作者： 刊期： 2015年第11期

Objective:To explore the correlation of expressions of MDR1/P-gp and CerbB-2 in the colorectal cancer tissues and their clinical significance.Methods:A total of 86 colorectal cancer tissues specimens were included in the study. The immunohistochemical S-P method was used to detect the expressions of P-gp and CerbB-2, and their correlations were analyzed.Results:The positive rates of the expressions of MDR1/P-gp and CerbB-2 were 36% (31/86) and 28% (24/86), respectively. The positive expression rate of CerbB-2 in the colorectal cancer tissues at a clinical stage of III was significantly higher than that at stage I and II. The positive expression rates of MDR1/P-gp and CerbB-2 in the colorectal cancer tissues with axillary lymph node metastasis were significantly higher than those in the tissues without axillary lymph node metastasis. In the P-gp positive expression group, the positive rate of CerbB-2 was 75.5% (37/49), while the negative rate of CerbB-2 was 24.48% (12/49), and the difference was statistically significant (P<0.01). P-gp had a certain positive correlation with the positive expression rate of CerbB-2. Conclusions:P-gp and CerbB-2 play a certain role in the occurrence of multidrug resistance of colorectal cancer, and their expressions are correlated; therefore, the combination detection can provide an evidence for the chemotherapy choice of colorectal cancer.

作者： 刊期： 2015年第11期

Objective:To explore the effect and mechanism of ganglioside for hypoxia injury in newborn.Methods:A total of 200 cases of newborn patients in our hospital were analyzed. All the patients were divided into 4 groups, control and ganglioside groups with low, medium and high dose. The neonatal behavioral neurological assessment scores were counted in each group and the serum inflammatory factors and transforming growth factor-β (TGFβ)/Smad parameters were detected.Results:The neonatal behavioral neurological assessment scores were increased after ganglioside treatment and the serum interleukin (IL) 2/10, hypoxia inducible factorαand Smad1, Smad3 as well as TGFβ was decreased dramatically when compared with control group.Conclusions:Ganglioside exerts an effective effect on cerebral hypoxia in newborn mainly by inhibiting the TGFβ/Smad signaling pathway.

作者： 刊期： 2015年第11期

Objective:To study the miRNA-21 content in cervical cancer tissue and analyze its downstream target molecules.Methods:Patients with different FIGO stages of cervical cancer and healthy subjects were selected, cervical cancer tissue and normal cervical tissue were collected, and contents of miRNA-21 and apoptotic genes were detected; cervical cancer SiHa cells were cultured, miRNA-21 mimics and inhibitors were transfected, and then apoptotic gene contents were detected.Results:miRNA-21 contents in different stages of cervical cancer tissue were all higher than those in normal cervical tissue, mRNA contents of p16ink4a, ASPP1, Fas and GRIM-19 were lower than those in normal tissue, and mRNA contents of p16ink4a, ASPP1, Fas and GRIM-19 were negatively correlated with miRNA-21 contents; after miRNA-21 mimics were transfected, mRNA contents of p16ink4a, ASPP1, Fas and GRIM-19 significantly decreased, and after miRNA-21 inhibitors were transfected, mRNA contents of p16ink4a, ASPP1, Fas and GRIM-19 significantly increased.Conclusion:miRNA-21 contents in cervical cancer tissue significantly increase; downstream target genes of this miRNA may be apoptotic genes p16ink4a, ASPP1, Fas and GRIM-19.

作者： 刊期： 2015年第11期

Objective:To discuss the clinical value of Serum TSH combined with 3 kinds of VEGF (VEGF-C, VEGF-D and VEGFR-3) determination in the early diagnosis of papillary thyroid carcinoma (PTC). Method:Selected 37 cases of patients with thyroid benign tumor (Benign group) and 37 cases of patients with PTC (PTC group), then collected the serum of these both groups, to determine the TSH, VEGF-C, VEGF-D and VEGFR-3 levels of all cases by chemiluminescence immunoassay and enzyme linked immunosorbent assay respectively. Through Logistic model, to calculate the curve area of TSH combined with 3 kinds of VEGF.Results:PTC group: VEGF-C, VEGFR-3 and TSH levels were obviously higher than that in Benign group (P<0.05); and VEGF-C, VEGFR-3 and TSH levels inⅢ-Ⅳ period patients were obviously higher than that in I-Ⅱ period patients (P<0.05); AUC area of VEGF-C, VEGFR-3 and TSH were respectively 0.805, 0.736 and 0.710, reached to significance level (P<0.05); AUC area of combined diagnosis was 0.859.Conclusion:VEGF-C, VEGFR-3 and TSH between papillary thyroid carcinoma and thyroid benign tumor had significant difference. Combined determination could improve the early diagnose rate of PTC, and could be regarded as one of the important auxiliary index of PTC early diagnosis.

作者： 刊期： 2015年第11期

Objective:To explore the clinical effect and significance of adoptive immunotherapy of dendritic cell and cytokine-induced killer cell (DC-CIK) combined with the gamma knife in the treatment of middle and advanced hepatic carcinoma.Methods:42 patients with the middle and advanced primary hepatic carcinoma were randomly divided into two groups: 20 cases in the combination group were given the adoptive immunotherapy of DC-CIK cells and gamma knife radiotherapy; 22 cases in the control group were only given the gamma knife radiotherapy. The short-term effect, quality of life, overall survival and toxic and side effects were compared between two groups after the operation.Results: 3 months after the treatment, the short-term effect of combination group and control group was 70% and 54.5% respectively (P<0.05). Patients in the combination group performed better in the overall survival, change of T-cell subsets, PS score, decrease rate of AFP and degree of liver function than the control group, while the adiodermatitis at II and over and bone marrow suppression were also better than the control group. Conclusion:The adoptive immunotherapy of DC-CIK cells combined with the gamma knife in the treatment of middle and advanced hepatic carcinoma can prolong the overall survival, improve the quality of life, reduce the toxic and side effect and effectively promote the short-term clinical effect for patients.

作者： 刊期： 2015年第11期

Objective:To study the expression of aminopeptidase N (CD13) in renal carcinoma and the effect of CD13 expression vector transfection on biological behavior of cancer cells.Methods:Renal carcinoma tissue and normal kidney tissue were collected and APN (CD13) contents in tissue were detected; renal carcinoma cell lines kevt-3 were cultured, 0 μg/mL, 10 μg/mL, 20 μg/mL, 40 μg/mL and 80 μg/mL of CD13 expression vector were transfected, and then migration ability, ATP generation capacity, and mRNA contents of migration and angiogenesis genes in cells were detected.Results:mRNA contents of APN in renal carcinoma tissue were higher than those in normal kidney tissue; the higher the clinical stage and pathological grade were, the higher the mRNA contents of APN in renal carcinoma tissue were; mRNA levels of APN in renal carcinoma tissue with lymph node metastasis were higher than those without lymph node metastasis;CD13 expression vector transfection could dose-dependently increase kevt-3 cell migration rate, ATP generation amount as well as mRNA contents of VEGF, HIF-1α, MMP9 and MMP10.Conclusion: Expression of aminopeptidase N (CD13) in renal carcinoma tissue abnormally increases; overexpression of CD13 can promote renal carcinoma cell migration and increase ATP generation as well as VEGF, HIF-1, MMP9 and MMP10 expression.

作者： 刊期： 2015年第11期

Objective:To explore the influence of inflammation factors and intestinal mucosal barrier function on severe acute pancreatitis patients treated with bifidobacterium triple viable capsules.Methods:88 cases severe acute pancreatitis patients were divided into observation group and control group according to admission order, 44 cases in each group, all patients were given conventional treatment, on this base, patients in observation group were treated by oral bifidobacterium triple viable capsules, they were treated for one week, detected the serum inflammatory factors: the tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6) and C-reactive protein (CRP) and intestinal mucosal barrier function index: endotoxin, diamine oxidase (DAO), D-lactic acid, urinary lactulose/mannitol (L/M) in the two groups before and after treatment.Results:The levels of TNF-α, IL-6 and CRP in two groups after treatment were significantly reduced than before treatment, and after treatment the levels of TNF-α, IL-6 and CRP in observation group was significantly lower than that of the control group, all the difference was statistically significant; The levels of endotoxin, DA, D-lactic acid, L/M in two groups after treatment were significantly reduced than before treatment, and the levels of endotoxin, DA, D-lactic acid,L/M after treatment in observation group was significantly lower than that of the control group, the difference was statistically significant.Conclusion: Based on conventional treatment combined bifidobacterium triple viable capsules can significantly reduce inflammation in patients with severe acute pancreatitis, it can protect the intestinal mucosal barrier function, and has important clinical significance.

作者： 刊期： 2015年第11期

Objective:To study the role of miR-29b in Docetaxel caused dampened metastasis of gastric cancer cells.Methods: After treated by Docetaxel, the invasion and migration of SGC7901 cell were studied by Transwell and Wound Healing Assay. miR-29b levels was detected by qRT-PCR and its targets were detected by Western blot after SGC7901 cell was treated by Docetaxel. When transfected by miR-29b mimic, the invasion and migration of SGC7901 cell were studied by Transwell and Wound Healing Assay. Similarly, when treated by Docetaxel and anti-miR-29b simultaneously, the invasion and migration of SGC7901 cell were also studied by Transwell and Wound Healing Assay.Results:Docetaxel could inhibit the invasion and migration of SGC7901 cell; miR-29b was upregulated by Docetaxel in SGC7901 cell; miR-29b could inhibit the invasion and migration of SGC7901 cell; When miR-29b could not be upregulated by Docetaxel, the effects of Docetaxel on invasion and migration of SGC7901 cell would be dampened.Conclusion:Docetaxel upregulated miR-29b contributes to dampened metastasis of gastric cancer cells.

作者： 刊期： 2015年第11期